Employing Response Surface Methodology for the Optimization of Ultrasound Assisted Extraction of Lutein and β-Carotene from SpinachReportar como inadecuado




Employing Response Surface Methodology for the Optimization of Ultrasound Assisted Extraction of Lutein and β-Carotene from Spinach - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

1

Department of Plant, Soil and Agricultural Systems, Southern Illinois University, Carbondale, IL 62901, USA

2

Department of Food Science and Biotechnology, College of Agriculture, University of Basrah, Basrah 61004, Iraq

3

SIUC Mass Spectrometry Facility, Department of Chemistry and Biochemistry, Southern Illinois University, Carbondale, IL 62901, USA





*

Author to whom correspondence should be addressed.



Academic Editors: Nigel Brunton, Mohammad Hossain and Dilip Rai

Abstract The extraction of lutein and β-carotene from spinach Spinacia oleracea L. leaves is important to the dietary supplement industry. A Box-Behnken design and response surface methodology RSM were used to investigate the effect of process variables on the ultrasound-assisted extraction UAE of lutein and β-carotene from spinach. Three independent variables, extraction temperature °C, extraction power % and extraction time min were studied. Thin-layer chromatography TLC followed by UV visualization and densitometry was used as a simple and rapid method for both identification and quantification of lutein and β-carotene during UAE. Methanol extracts of leaves from spinach and authentic standards of lutein and β-carotene were separated by normal-phase TLC with ethyl acetate-acetone 5:4 v-v as the mobile phase. In this study, the combination of TLC, densitometry, and Box–Behnken with RSM methods were effective for the quantitative analysis of lutein and β-carotene from spinach extracts. The resulting quadratic polynomial models for optimizing lutein and β-carotene from spinach had high coefficients of determination of 0.96 and 0.94, respectively. The optimal UAE settings for output of lutein and β-carotene simultaneously from spinach extracts were an extraction temperature of 40 °C, extraction power of 40% 28 W-cm3 and extraction time of 16 min. The identity and purity of each TLC spot was measured using time-of-flight mass spectrometry. Therefore, UAE assisted extraction of carotenes from spinach can provide a source of lutein and β-carotene for the dietary supplement industry. View Full-Text

Keywords: lutein; β-carotene; spinach; ultrasound; TLC; densitometry; mass spectrometry lutein; β-carotene; spinach; ultrasound; TLC; densitometry; mass spectrometry





Autor: Ammar Altemimi 1,2,* , David A. Lightfoot 1, Mary Kinsel 3 and Dennis G. Watson 1

Fuente: http://mdpi.com/



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