Anti-Inflammatory Effect of Wogonin on RAW 264.7 Mouse Macrophages Induced with Polyinosinic-Polycytidylic AcidReportar como inadecuado




Anti-Inflammatory Effect of Wogonin on RAW 264.7 Mouse Macrophages Induced with Polyinosinic-Polycytidylic Acid - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Department of Pathology, College of Korean Medicine, Gachon University, Seongnam 461-701, Korea





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Academic Editor: Jean Jacques Vanden Eynde

Abstract Wogonin 5,7-dihydroxy-8-methoxyflavone is an active flavonoid compound originally isolated from Scutellaria radix, which has been used to treat lung inflammation in Korea, China, and Japan. Wogonin has been known to inhibit inducible nitric oxide synthase and have the anti-tumor properties. However, the effects of wogonin on virus-induced macrophages are not fully reported. In this study, the anti-inflammatory effect of wogonin on double-stranded RNA dsRNA-induced macrophages was examined. Wogonin restored the cell viability in dsRNA polyinosinic-polycytidylic acid-induced RAW 264.7 mouse macrophages at concentrations of up to 50 μM. Wogonin significantly inhibited the production of nitric oxide, IL-1α, IL-1β, IL-6, IL-10, IP-10, G-CSF, GM-CSF, LIF IL-6 class cytokine, LIX-CXCL5, MCP-1, M-CSF, MIP-1α, MIP-1β, MIP-2, RANTES-CCL5, TNF-α, and VEGF as well as calcium release and mRNA expression of signal transducer and activated transcription 1 STAT1 and STAT3 in dsRNA-induced RAW 264.7 cells P < 0.05. In conclusion, wogonin has anti-inflammatory properties related with its inhibition of nitric oxide, cytokines, chemokines, and growth factors in dsRNA-induced macrophages via the calcium-STAT pathway. View Full-Text

Keywords: wogonin; dsRNA; inflammation; macrophages; nitric oxide; cytokine; calcium; STAT1; STAT3 wogonin; dsRNA; inflammation; macrophages; nitric oxide; cytokine; calcium; STAT1; STAT3





Autor: Ji Young Lee and Wansu Park *

Fuente: http://mdpi.com/



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