Isolation of an Angiotensin I-Converting Enzyme Inhibitory Protein with Antihypertensive Effect in Spontaneously Hypertensive Rats from the Edible Wild Mushroom Leucopaxillus tricolorReport as inadecuate




Isolation of an Angiotensin I-Converting Enzyme Inhibitory Protein with Antihypertensive Effect in Spontaneously Hypertensive Rats from the Edible Wild Mushroom Leucopaxillus tricolor - Download this document for free, or read online. Document in PDF available to download.

1

State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100193, China

2

Institute of Biotechnology and Germplasmic Resource, Yunnan Academy of Agricultural Science, Kunming 650223, China

3

College of Food Science and Technology, Nanjing Agricultural University, Weigang, Nanjing 210095, China

4

School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China





*

Authors to whom correspondence should be addressed.



Academic Editor: Nancy D. Turner

Abstract An 86-kDa homodimeric angiotensin I-converting enzyme ACE inhibitory protein designated as LTP was isolated from fruit bodies of the mushroom Leucopaxillus tricolor. The isolation procedure involved ultrafiltration through a membrane with a molecular weight cutoff of 10-kDa, ion exchange chromatography on Q-Sepharose, and finally fast protein liquid chromatography-gel filtration on Superdex 75. LTP exhibited an IC50 value of 1.64 mg∙mL−1 for its ACE inhibitory activity. The unique N-terminal amino acid sequence of LTP was disclosed by Edman degradation to be DGPTMHRQAVADFKQ. In addition, seven internal sequences of LTP were elucidated by liquid chromatography-tandem mass spectrometry LC-MS-MS analysis. Results of the Lineweaver-Burk plot suggested that LTP competitively inhibited ACE. Both LTP and the water extract of L. tricolor exhibited a clear antihypertensive effect on spontaneously hypertensive rats. View Full-Text

Keywords: Leucopaxillus tricolor; ACE inhibitory protein; inhibitory pattern; spontaneously hypertensive rat Leucopaxillus tricolor; ACE inhibitory protein; inhibitory pattern; spontaneously hypertensive rat





Author: Xueran Geng 1, Guoting Tian 2, Weiwei Zhang 1, Yongchang Zhao 2, Liyan Zhao 3, Mansok Ryu 1, Hexiang Wang 1,* and Tzi Bun Ng 4,*

Source: http://mdpi.com/



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