Antioxidant Capacities and Analysis of Phenolic Compounds in Three Endemic Nolana Species by HPLC-PDA-ESI-MSReport as inadecuate




Antioxidant Capacities and Analysis of Phenolic Compounds in Three Endemic Nolana Species by HPLC-PDA-ESI-MS - Download this document for free, or read online. Document in PDF available to download.

1

Laboratorio de Productos Naturales, Departamento de Química, Facultad de Ciencias Básicas, Universidad de Antofagasta, Av. Coloso S-N, Antofagasta 1240000, Chile

2

Facultad de Ciencias de la Salud, Universidad Arturo Prat, Iquique 1100000, Chile

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Departamento de Química, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago 7800024, Chile

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Departamento de Ciencias Químicas, Universidad Andres Bello, Campus Viña del Mar, Quillota 980, Viña del Mar 2520000, Chile





*

Author to whom correspondence should be addressed.



Academic Editor: Derek J. McPhee

Abstract The antioxidant features, polyphenolic composition and chromatographic fingerprints of the aerial parts from three Chilean endemic plants from the Paposo Valley located on the cost of the Atacama Desert were investigated for the first time using high pressure liquid chromatography coupled with photodiode array detector and electrospray ionization mass analysis HPLC-PDA-ESI-MS and spectroscopic methods. The phenolic fingerprints obtained for the plants were compared and correlated with the antioxidant capacities measured by the bleaching of the DPPH radical, the ferric reducing antioxidant power FRAP and quantification of the total content of phenolics and flavonoids measured by spectroscopic methods. Thirty phenolics were identified for the first time for these species, mostly phenolic acids, flavanones, flavonols and some of their glycoside derivatives, together with three saturated fatty acids stearic, palmitic and arachidic acids. Nolana ramosissima showed the highest antioxidant activity 26.35 ± 1.02 μg-mL, 116.07 ± 3.42 μM Trolox equivalents-g dry weight and 81.23% ± 3.77% of inhibition in the DPPH, FRAP and scavenging activity SA assays, respectively, followed by N. aplocaryoides 85.19 ± 1.64 μg-mL, 65.87 ± 2.33 μM TE-g DW and 53.27% ± 3.07% and N. leptophylla 124.71 ± 3.01, 44.23 ± 5.18 μM TE-g DW and 38.63% ± 1.85%. View Full-Text

Keywords: Chilean plants; Atacama Desert; phenolics; antioxidant capacity; HPLC-MS; Paposo Valley; Nolana Chilean plants; Atacama Desert; phenolics; antioxidant capacity; HPLC-MS; Paposo Valley; Nolana





Author: Mario J. Simirgiotis 1,* , Julio Benites 2, Carlos Areche 3 and Beatriz Sepúlveda 4

Source: http://mdpi.com/



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