Induction of Mitochondrial Dependent Apoptosis in Human Leukemia K562 Cells by Meconopsis integrifolia: A Species from Traditional Tibetan MedicineReport as inadecuate




Induction of Mitochondrial Dependent Apoptosis in Human Leukemia K562 Cells by Meconopsis integrifolia: A Species from Traditional Tibetan Medicine - Download this document for free, or read online. Document in PDF available to download.

1

Co-Innovation Center for Qinba Regions Sustainable Development, College of Life Sciences, Shaanxi Normal University, No. 620, West Changan Avenue, Changan District, 710119 Xian, China

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College of Life Sciences, Qinghai Normal University, No.38, West Wusi Road, 810008 Xining, China





*

Author to whom correspondence should be addressed.



Academic Editor: Jean Jacques Vanden Eynde

Abstract Objectives: Meconopsis integrifolia M. integrifolia is one of the most popular members in Traditional Tibetan Medicine. This study aimed to investigate the anticancer effect of M. integrifolia and to detect the underlying mechanisms of these effects. Methods: 3-4,5-dimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide MTT assay and trypan blue assay were used to evaluate the cytotoxicity of M. integrifolia. Changes in cell nuclear morphology and reactive oxygen species ROS level were observed by fluorescent microscopy. Apoptosis ratio, DNA damage and mitochondrial membrane potential MMP loss were analyzed by flow cytometry. Western blotting assay was adopted to detect the proteins related to apoptosis. Immunofluorescence was used to observe the release of cytochrome C. Results: The obtained data revealed that M. integrifolia could significantly inhibit K562 cell viability, mainly by targeting apoptosis induction and cell cycle arrest in G2-M phase. Collapse in cell morphology, chromatin condensation, DNA damage and ROS accumulation were observed. Further mechanism detection revealed that mitochondrion might be a key factor in M. integrifolia-induced apoptosis. Conclusions: M. integrifolia could induce mitochondria mediated apoptosis and cell cycle arrest in G2-M phase with little damage to normal cells, suggesting that M. integrifolia might be a potential and efficient anticancer agent that deserves further investigation. View Full-Text

Keywords: M. integrifolia ethanol extract; K562 cells; apoptosis; mitochondria; G2-M phase arrest M. integrifolia ethanol extract; K562 cells; apoptosis; mitochondria; G2-M phase arrest





Author: Jianping Fan 1,2, Pan Wang 1, Xiaobing Wang 1, Wei Tang 1, Chunliang Liu 1, Yaqin Wang 1, Wenjuan Yuan 1, Lulu Kong 1 and Quanhong Liu 1,*

Source: http://mdpi.com/



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