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Faculty of Chemical Technology and Engineering, University of Science and Technology, UTP, Bydgoszcz 85-326, Poland





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Academic Editor: Derek J. McPhee

Abstract Steady state fluorescence measurements have been used for the investigation of interaction between the bovine serum albumin BSA and fluorescence probes: 3-hydroxy-2,4- bis3-methyl-1,3-benzoxazol-23H-ylidenemethylcyclobut-2-en-1-one SQ6, 3-hydroxy- 2,4-bis3-methyl-1,3-benzothiazol-23H-ylidenemethylcyclobut-2-en-1-one SQ7 and 3-hydroxy-2,4-bis1,3,3-trimethyl-1,3-dihydro-2H-indol-2-ylidenemethylcyclobut-2-en-1-one SQ8. The binding constant between bovine serum albumin and squarine dyes has been determined by using both the Benesi-Hildebrand and Stern-Volmer equations. The negative value of free energy change indicates the existence of a spontaneous complexation process of BSA with squarine dyes. View Full-Text

Keywords: bovine serum albumin; squarine dyes; fluorescence probe; biomolecule determination; fluorescence method bovine serum albumin; squarine dyes; fluorescence probe; biomolecule determination; fluorescence method





Autor: Katarzyna Jurek * , Janina Kabatc, Katarzyna Kostrzewska and Marlena Grabowska

Fuente: http://mdpi.com/



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