The Complete Chloroplast Genome of Capsicum annuum var. glabriusculum Using Illumina SequencingReportar como inadecuado




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1

National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration, Jeonju 560-500, Korea

2

Department of Forest Genetic Resources, Korea Forest Research Institute, Suwon 441-350, Korea





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Author to whom correspondence should be addressed.



Academic Editor: Derek J. McPhee

Abstract Chloroplast cp genome sequences provide a valuable source for DNA barcoding. Molecular phylogenetic studies have concentrated on DNA sequencing of conserved gene loci. However, this approach is time consuming and more difficult to implement when gene organization differs among species. Here we report the complete re-sequencing of the cp genome of Capsicum pepper Capsicum annuum var. glabriusculum using the Illumina platform. The total length of the cp genome is 156,817 bp with a 37.7% overall GC content. A pair of inverted repeats IRs of 50,284 bp were separated by a small single copy SSC; 18,948 bp and a large single copy LSC; 87,446 bp. The number of cp genes in C. annuum var. glabriusculum is the same as that in other Capsicum species. Variations in the lengths of LSC; SSC and IR regions were the main contributors to the size variation in the cp genome of this species. A total of 125 simple sequence repeat SSR and 48 insertions or deletions variants were found by sequence alignment of Capsicum cp genome. These findings provide a foundation for further investigation of cp genome evolution in Capsicum and other higher plants. View Full-Text

Keywords: Capsicum; bird pepper; chloroplast; DNA sequencing Capsicum; bird pepper; chloroplast; DNA sequencing





Autor: Sebastin Raveendar 1, Young-Wang Na 1, Jung-Ro Lee 1, Donghwan Shim 2, Kyung-Ho Ma 1, Sok-Young Lee 1 and Jong-Wook Chung 1,*

Fuente: http://mdpi.com/



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