Separation of Polyphenols and Caffeine from the Acetone Extract of Fermented Tea Leaves Camellia sinensis Using High-Performance Countercurrent ChromatographyReport as inadecuate




Separation of Polyphenols and Caffeine from the Acetone Extract of Fermented Tea Leaves Camellia sinensis Using High-Performance Countercurrent Chromatography - Download this document for free, or read online. Document in PDF available to download.

1

College of Pharmacy, Integrated Research Institute of Pharmaceutical Sciences, The Catholic University of Korea, Bucheon 420-743, Korea

2

AmorePacific R&D Unit, 314-1 Bora-dong, Giheung-gu, Yongin-si, Gyeonggi-do 449-729, Korea



These authors contributed equally to this work.





*

Author to whom correspondence should be addressed.



Academic Editor: Derek J. McPhee

Abstract Leaves from Camellia sienensis are a popular natural source of various beverage worldwide, and contain caffeine and polyphenols derived from catechin analogues. In the current study, caffeine CAF, 1 and three tea polyphenols including −-epigallocatechin 3-O-gallate EGCg, 2, −-gallocatechin 3-O-gallate GCg, 3, and −-epicatechin 3-O-gallate ECg, 4 were isolated and purified by flow-rate gradient high-performance countercurrent chromatography HPCCC using a two-phase solvent system composed of n-hexane–ethyl acetate–methanol–water 1:9:1:9, v-v. Two hundred milligrams of acetone-soluble extract from fermented C. sinensis leaves was separated by HPCCC to give 1 25.4 mg, 2 16.3 mg, 3 11.1 mg and 4 4.4 mg with purities over 98%. The structures of 1–4 were elucidated by QTOF-MS, as well as 1H- and 13C-NMR, and the obtained data were compared to the previously reported values. View Full-Text

Keywords: Camellia sinensis; tea-polyphenols; high-performance countercurrent chromatography Camellia sinensis; tea-polyphenols; high-performance countercurrent chromatography





Author: Soo Jung Choi 1,†, Yong Deog Hong 2,†, Bumjin Lee 2, Jun Seong Park 2, Hyun Woo Jeong 2, Wan Gi Kim 2, Song Seok Shin 2 and Kee Dong Yoon 1,*

Source: http://mdpi.com/



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