Antibacterial Activity of Protocatechuic Acid Ethyl Ester on Staphylococcus aureus Clinical Strains Alone and in Combination with Antistaphylococcal DrugsReport as inadecuate




Antibacterial Activity of Protocatechuic Acid Ethyl Ester on Staphylococcus aureus Clinical Strains Alone and in Combination with Antistaphylococcal Drugs - Download this document for free, or read online. Document in PDF available to download.

Department of Microbiology and Virology, School of Pharmacy and Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, ul. Jagiellońska 4, 41-200 Sosnowiec, Poland



These authors contributed equally to this work.





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Academic Editor: Peter J. Rutledge

Abstract The aim of the presented study was to examine in vitro the antibacterial activity of protocatechuic acid ethyl ester ethyl 3,4-dihydroxybenzoate, EDHB against Staphylococcus aureus clinical isolates alone and in the combination with four selected antibiotics. The EDHB antimicrobial activity was tested against twenty S. aureus strains isolated from the clinical samples, and three reference strains. The phenotypes and genotypes of resistance to methicillin for the tested strains were defined as well as the phenotypic resistance to macrolides, lincosamides and streptogramin B MLSB. EDHB displayed diverse activity against examined S. aureus strains with the minimal inhibitory concentration MIC within the range from 64 to 1024 µg-mL. Addition of ¼ MIC of EDHB into the Mueller-Hinton Agar MHA resulted in augmented antibacterial effect in the presence of clindamycin. In the case of cefoxitin no synergistic effect with EDHB was noted. For erythromycin and vancomycin the decrease of mean MICs in the presence of EDHB was observed but did not reach statistical significance. The results of the present study showed that in vitro EDHB possesses antibacterial activity against S. aureus clinical strains and triggers a synergistic antimicrobial effect with clindamycin and to the lesser extent with erythromycin and vancomycin. View Full-Text

Keywords: Staphylococcus aureus; protocatechuic acid ethyl ester; antibacterial activity; synergistic effect Staphylococcus aureus; protocatechuic acid ethyl ester; antibacterial activity; synergistic effect





Author: Maria Miklasińska †, Małgorzata Kępa †, Robert D. Wojtyczka, Danuta Idzik, Anna Zdebik, Kamila Orlewska and Tomasz J. Wąsik *

Source: http://mdpi.com/



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