Optimization of Micellar Electrokinetic Chromatography Method for the Simultaneous Determination of Seven Hydrophilic and Four Lipophilic Bioactive Components in Three Salvia SpeciesReportar como inadecuado




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1

State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau 999078, China

2

Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China



These authors contributed equally to this work.





*

Authors to whom correspondence should be addressed.



Academic Editor: Derek J. McPhee

Abstract A micellar electrokinetic chromatography MEKC method was developed for the simultaneous determination of seven hydrophilic phenolic acids and four lipophilic tanshinones in three Salvia species. In normal MEKC mode using SDS as surfactant, the investigated 11 compounds could not be well separated. Therefore, several buffer modifiers including β-cyclodextrins β-CD, ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborate bmimBF4 and organic solvents have been added to the buffer solution to improve the separation selectivity. Under the optimized conditions BGE, 15 mM sodium tetraborate with 10 mM SDS, 5 mM β-CD, 10 mM bmimBF4 and 15% ACN v-v as additives; buffer pH, 9.8; voltage, 20 kV; temperature, 25 °C, the 11 investigated analytes could achieve baseline separation in 34 min. The proposed MEKC was additionally validated by evaluating the linearity R2 ≥ 0.9965, LODs 0.27–1.39 μg·mL–1, and recovery 94.26%–105.17%, demonstrating this method was reproducible, accurate and reliable. Moreover, the contents of the 11 compounds in three Salvia species, including S. miltiorrhiza, S. przewalskii and S. castanea were analyzed. The result showed that the established MEKC method was simple and practical for the simultaneous determination of the hydrophilic and lipophilic bioactive components in Salvia species, which could be used to effectively evaluate the quality of these valued medicinal plants. View Full-Text

Keywords: micellar electrokinetic chromatography; buffer modifiers; phenolic acids; tanshinones; Salvia species micellar electrokinetic chromatography; buffer modifiers; phenolic acids; tanshinones; Salvia species





Autor: Jiliang Cao 1,†, Ji Hu 1,†, Jinchao Wei 1, Baocai Li 2,* , Mi Zhang 2, Cheng Xiang 2 and Peng Li 1,*

Fuente: http://mdpi.com/



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