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1

Facultad de Química, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico

2

Instituto de Biología, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico





*

Author to whom correspondence should be addressed.



Academic Editor: Isabel C. F. R. Ferreira

Abstract The α-glucosidase inhibitory activity of an aqueous extract and compounds from the aerial parts of V. corymbosa was demonstrated with yeast and rat small intestinal α-glucosidases. The aqueous extract inhibited yeast α-glucosidase with a half maximal inhibitory concentration IC50 of 28.6 μg-mL. Bioassay-guided fractionation of the extract led to the isolation of several compounds, including one cyanogenic glycoside prunasin 1, five flavonoids −-epi-catechin 2, hyperoside 3, isoquercetin 4, quercitrin 5 and quercetin-3-O-6′′-benzoyl-β-galactoside 6 and two simple aromatic compounds picein 7 and methylarbutin 8. The most active compound was 6 with IC50 values of 30 μM in the case of yeast α-glucosidase, and 437 μM in the case of the mammalian enzyme. According to the kinetic analyses performed with rat and yeast enzymes, this compound behaved as mixed-type inhibitor; the calculated inhibition constants Ki were 212 and 50 μM, respectively. Molecular docking analyses with yeast and mammalian α-glucosidases revealed that compound 6 bind differently to these enzymes. Altogether, the results of this work suggest that preparations of V. corymbosa might delay glucose absorption in vivo. View Full-Text

Keywords: diabetes; yeast and rat α-glucosidases; Vauquelinia corymbosa diabetes; yeast and rat α-glucosidases; Vauquelinia corymbosa





Autor: Laura Flores-Bocanegra 1, Araceli Pérez-Vásquez 1, Mariana Torres-Piedra 1, Robert Bye 2, Edelmira Linares 2 and Rachel Mata 1,*

Fuente: http://mdpi.com/



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