Phenyllactic Acid from Lactobacillus plantarum PromotesAdipogenic Activity in 3T3-L1 Adipocyte via Up-Regulationof PPAR-γ2Report as inadecuate




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1

Grassland and Forage Division, National Institute of Animal Science, RDA, Seonghwan-Eup, Cheonan-Si, Chungnam 330801, Korea

2

The United Graduate School of Agricultural Sciences, Tottori University, Tottori-Shi 6808553, Japan

3

Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia

4

Department of Biotechnology, PRIST University, Tamilnadu 613403, India



These authors contributed equally to this work.





*

Author to whom correspondence should be addressed.



Academic Editor: Derek J. McPhee

Abstract Synthetic drugs are commonly used to cure various human ailments at present. However, the uses of synthetic drugs are strictly regulated because of their adverse effects. Thus, naturally occurring molecules may be more suitable for curing disease without unfavorable effects. Therefore, we investigated phenyllactic acid PLA from Lactobacillus plantarum with respect to its effects on adipogenic genes and their protein expression in 3T3-L1 pre-adipocytes by qPCR and western blot techniques. PLA enhanced differentiation and lipid accumulation in 3T3-L1 cells at the concentrations of 25, 50, and 100 μM. Maximum differentiation and lipid accumulation were observed at a concentration of 100 μM of PLA, as compared with control adipocytes p < 0.05. The mRNA and protein expression of PPAR-γ2, C-EBP‑α, adiponectin, fatty acid synthase FAS, and SREBP-1 were increased by PLA treatment as compared with control adipocytes p < 0.05. PLA stimulates PPAR-γ mRNA expression in a concentration dependent manner, but this expression was lesser than agonist 2.83 ± 0.014 fold of PPAR-γ2. Moreover, PLA supplementation enhances glucose uptake in 3T3-L1 pre-adipocytes 11.81 ± 0.17 mM compared to control adipocytes, but this glucose uptake was lesser than that induced by troglitazone 13.75 ± 0.95 mM and insulin treatment 15.49 ± 0.20 mM. Hence, we conclude that PLA treatment enhances adipocyte differentiation and glucose uptake via activation of PPAR-γ2, and PLA may thus be the potential candidate for preventing Type 2 Diabetes Mellitus T2DM. View Full-Text

Keywords: phenyllactic acid; 3T3-L1 pre-adipocyte; adipocyte differentiation; troglitazone; PPAR-γ antagonist; glucose uptake; type-2 diabetes phenyllactic acid; 3T3-L1 pre-adipocyte; adipocyte differentiation; troglitazone; PPAR-γ antagonist; glucose uptake; type-2 diabetes





Author: Soundharrajan Ilavenil 1,†, Da Hye Kim 2,†, Mariadhas Valan Arasu 3, Srisesharam Srigopalram 1, Ravikumar Sivanesan 4 and Ki Choon Choi 1,*

Source: http://mdpi.com/



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