Optimization of Ultrasonic-Assisted Extraction of Flavonoid Compounds and Antioxidants from Alfalfa Using Response Surface MethodReportar como inadecuado




Optimization of Ultrasonic-Assisted Extraction of Flavonoid Compounds and Antioxidants from Alfalfa Using Response Surface Method - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China





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Academic Editor: Mohammad Hossain

Abstract Ultrasonic-assisted extraction UAE was used to extract flavonoid-enriched antioxidants from alfalfa aerial part. Response surface methodology RSM, based on a four-factor, five-level central composite design CCD, was employed to obtain the optimal extraction parameters, in which the flavonoid content was maximum and the antioxidant activity of the extracts was strongest. Radical scavenging capacity of the extracts, which represents the amounts of antioxidants in alfalfa, was determined by using 2,2′-azino-bis 3-ethylbenzothiazoline-6-sulphonicacid ABTS and 2,2′-diphenyl-1-picrylhydrazyl DPPH methods. The results showed good fit with the proposed models for the total flavonoid extraction R2 = 0.9849, for the antioxidant extraction assayed by ABTS method R2 = 0.9764, and by DPPH method R2 = 0.9806. Optimized extraction conditions for total flavonoids was a ratio of liquid to solid of 57.16 mL-g, 62.33 °C, 57.08 min, and 52.14% ethanol. The optimal extraction parameters of extracts for the highest antioxidant activity by DPPH method was a ratio of liquid to solid 60.3 mL-g, 54.56 °C, 45.59 min, and 46.67% ethanol, and by ABTS assay was a ratio of liquid to solid 47.29 mL-g, 63.73 °C, 51.62 min, and 60% ethanol concentration. Our work offers optimal extraction conditions for total flavonoids and antioxidants from alfalfa. View Full-Text

Keywords: alfalfa; flavonoid compounds; ultrasonic-assisted extraction; response surface methodology; antioxidant capacity alfalfa; flavonoid compounds; ultrasonic-assisted extraction; response surface methodology; antioxidant capacity





Autor: Chang-Liang Jing, Xiao-Fang Dong * and Jian-Ming Tong

Fuente: http://mdpi.com/



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