Glossogyne tenuifolia Extract Inhibits TNF-α-Induced Expression of Adhesion Molecules in Human Umbilical Vein Endothelial Cells via Blocking the NF-kB Signaling PathwayReport as inadecuate




Glossogyne tenuifolia Extract Inhibits TNF-α-Induced Expression of Adhesion Molecules in Human Umbilical Vein Endothelial Cells via Blocking the NF-kB Signaling Pathway - Download this document for free, or read online. Document in PDF available to download.

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Institute of Biotechnology and Chemical Engineering, I-Shou University, Kaohsiung 84001, Taiwan

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Division of Cardiology, Department of Internal Medicine, E-Da Hospital-I-Shou University, Kaohsiung 82445, Taiwan

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Department of Nutrition, I-Shou University, Kaohsiung 82445, Taiwan

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Division of Pulmonary Medicine, Department of Internal Medicine, E-Da Hospital-I-Shou University, Kaohsiung 82445, Taiwan

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Division of Cardiology, Department of Internal Medicine, National Taiwan University Hospital, Taipei 10002, Taiwan





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Academic Editor: Derek J. McPhee

Abstract Chronic inflammation plays a pivotal role in the development of atherosclerosis, where the pro-inflammatory cytokine-induced expression of endothelial adhesion molecules and the recruitment of monocytes are the crucial events leading to its pathogenesis. Glossogyne tenuifolia ethanol extract GTE is shown to have potent anti-inflammatory and antioxidant activities. We evaluated the effects of GTE and its major components, luteolin lut, luteolin-7-glucoside lut-7-g, and oleanolic acid OA on TNF-α-induced expression of adhesion molecules in human umbilical vein endothelial cells HUVECs. The results demonstrated that GTE, lut, and lut-7-g attenuated the expression of intercellular adhesion molecule-1 ICAM-1 and vascular cell adhesion molecule-1 VCAM-1 in TNF-α-activated HUVECs, and inhibited the adhesion of monocytes to TNF-α-activated HUVECs. The TNF-α-induced mRNA expression of ICAM-1 and VCAM-1 was also suppressed, revealing their inhibitory effects at the transcriptional level. Furthermore, GTE, lut, and lut-7-g blocked the TNF-α-induced degradation of nuclear factor-kB inhibitor IkB, an indicator of the activation of nuclear factor-kB NF-kB. In summary, GTE and its bioactive components were effective in preventing the adhesion of monocytes to cytokine-activated endothelium by the inhibition of expression of adhesion molecules, which in turn is mediated through blocking the activation and nuclear translocation of NF-kB. The current results reveal the therapeutic potential of GTE in atherosclerosis. View Full-Text

Keywords: Glossogyne tenuifolia; luteolin; luteolin-7-glucoside; adhesion molecule; nuclear factor-kB Glossogyne tenuifolia; luteolin; luteolin-7-glucoside; adhesion molecule; nuclear factor-kB





Author: Chin-Feng Hsuan 1,2, Hsia-Fen Hsu 3, Wei-Kung Tseng 2, Thung-Lip Lee 2, Yu-Feng Wei 4, Kwan-Lih Hsu 2, Chau-Chung Wu 5 and Jer-Yiing Houng 1,3,*

Source: http://mdpi.com/



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