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1

Department of Chemistry, Duke University, 124 Science Drive, Durham, NC 27708-0354, USA

2

Department of Chemistry, Carnegie Mellon University, Pittsburgh, PA 15213, USA

3

Department of Materials Science and Engineering, North Carolina State University, 911 Partners Way, Raleigh, NC 27695-7907, USA





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Authors to whom correspondence should be addressed.



Academic Editor: Ramon Eritja

Abstract DNA has shown great promise as a building material for self-assembling nanoscale structures. To further develop the potential of this technology, more methods are needed for functionalizing DNA-based nanostructures to increase their chemical diversity. Peptide nucleic acid PNA holds great promise for realizing this goal, as it conveniently allows for inclusion of both amino acids and peptides in nucleic acid-based structures. In this work, we explored incorporation of a positively charged PNA within DNA nanostructures. We investigated the efficiency of annealing a lysine-containing PNA probe with complementary, single-stranded DNA sequences within nanostructures, as well as the efficiency of duplex invasion and its dependence on salt concentration. Our results show that PNA allows for toehold-free strand displacement and that incorporation yield depends critically on binding site geometry. These results provide guidance for the design of PNA binding sites on nucleic acid nanostructures with an eye towards optimizing fabrication yield. View Full-Text

Keywords: peptide nucleic acid; oligonucleotide conjugates; modified oligonucleotides; DNA nanobiotechnology; nanomaterials peptide nucleic acid; oligonucleotide conjugates; modified oligonucleotides; DNA nanobiotechnology; nanomaterials





Autor: Ronnie O. Pedersen 1, Jing Kong 2, Catalina Achim 2,* and Thomas H. LaBean 3,*

Fuente: http://mdpi.com/



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