Antiproliferative and Apoptotic Activity of Chamaecyparis obtusa Leaf Extract against the HCT116 Human Colorectal Cancer Cell Line and Investigation of the Bioactive Compound by Gas Chromatography-Mass Spectrometry-Based MetabolomReportar como inadecuado




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1

College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea

2

Department of Food Science and Technology, Chung-Ang University, Anseong 456-756, Korea

3

Department of Food Science and Technology, Ewha Womans University, Seoul 120-750, Korea



These authors contributed equally to this work.





*

Author to whom correspondence should be addressed.



Academic Editor: Derek J. McPhee

Abstract Chamaecyparis obtusa CO belongs to the Cupressaceae family, and it is found widely distributed in Japan and Korea. In this study, the anti-proliferative activities of the methanol and water extracts of CO leaves against a human colorectal cancer cell line HCT116 were investigated. The methanol extract of CO leaves, at a concentration of 1.25 µg-mL, exhibited anti-proliferative activity against HCT116 cells, while displaying no cytotoxicity against Chang liver cells. Comparative global metabolite profiling was performed using gas chromatography-mass spectrometry coupled with multivariate statistical analysis, and it was revealed that anthricin was the major compound contributing to the anti-proliferative activity. The activation of c-Jun N-terminal kinases played a key role in the apoptotic effect of the methanol extract of CO leaves in HCT116 human colon cancer cells. These results suggest that the methanol extract and anthricin derived from CO leaves might be useful in the development of medicines with anti-colorectal cancer activity. View Full-Text

Keywords: Chamaecyparis obtusa; human colorectal cancer; metabolite profiling; gas chromatography-mass spectrometry; anthricin Chamaecyparis obtusa; human colorectal cancer; metabolite profiling; gas chromatography-mass spectrometry; anthricin





Autor: Hye-Youn Kim 1,†, Seul-Gi Lee 1,†, Taek-Joo Oh 1, Sa Rang Lim 1, So-Hyun Kim 1, Hong Jin Lee 2, Young-Suk Kim 3 and Hyung-Kyoon Choi 1,*

Fuente: http://mdpi.com/



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