Genetic Authentication of Gardenia jasminoides Ellis var. grandiflora Nakai by Improved RAPD-Derived DNA MarkersReportar como inadecuado




Genetic Authentication of Gardenia jasminoides Ellis var. grandiflora Nakai by Improved RAPD-Derived DNA Markers - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

1

Research Center for Preclinical Medicine, Sichuan Medical University, Luzhou 646000, China

2

State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau SAR 999078, China

3

Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran 14359-44711, Iran

4

Department of Biochemistry, School of Life Sciences & the State Key Laboratory of Medical Genetics, Central South University, Changsha 410013, China

5

Judicial Authentication Center, Sichuan Medical University, Luzhou 646000, China



These authors contributed equally to this work.





*

Author to whom correspondence should be addressed.



Academic Editor: Derek J. McPhee

Abstract The evergreen shrub, Gardenia jasminoides Ellis var. grandiflora Nakai is one of the most popular garden-plants, with significant ornamental importance. Here, we have cloned improved random amplified polymorphic DNA RAPD derived fragments into T-vector, and developed sequence-characterized amplified region SCAR markers. These markers have been deposited in GenBank database with the accession numbers KP641310, KP641311, KP641312 and KP641313 respectively. The BLAST search of database confirmed the novelty of these markers. The four SCAR markers, namely ZZH11, ZZH31, ZZH41 and ZZH51 can specifically recognize the genetic materials of G. jasminoides from other plant species. Moreover, SCAR marker ZZH31 can be used to distinguish G. jasminoides Ellis var. grandiflora Nakai from other G. jasminoides on the market. Together, this study has developed four stably molecular SCAR markers by improved RAPD-derived DNA markers for the genetic identification and authentication, and for ecological conservation of medicinal and ornamental plant G. jasminoides. View Full-Text

Keywords: G. jasminoides Ellis var. grandiflora Nakai; Gardenia jasminoides; random amplified polymorphic DNA; sequence-characterized amplified region; cloning; genetic authentication G. jasminoides Ellis var. grandiflora Nakai; Gardenia jasminoides; random amplified polymorphic DNA; sequence-characterized amplified region; cloning; genetic authentication





Autor: Zhiqiang Mei 1,†, Boxu Zhou 1,†, Chunli Wei 1,2,†, Jingliang Cheng 1, Saber Imani 1,3, Hanchun Chen 4 and Junjiang Fu 1,2,5,*

Fuente: http://mdpi.com/



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