Cyclovirobuxine D Inhibits Cell Proliferation and Induces Mitochondria-Mediated Apoptosis in Human Gastric Cancer CellsReportar como inadecuado




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1

School of Naval Architecture, Ocean and Civil Engineering, Shanghai Jiao Tong University, No. 800 Dongchuan Road, Shanghai 200240, China

2

Ministry of Education of China MOE Key Laboratory of Hydrodynamics, Shanghai Jiao Tong University, No. 800 Dongchuan Road, Shanghai 200240, China

3

Department of General Surgery, School of Medicine, Xinhua Hospital, Affiliated to Shanghai Jiao Tong University, Shanghai 200092, China

4

School of Pharmacy, Shanghai Jiao Tong University, No. 800 Dongchuan Road, Shanghai 200240, China

5

Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA 16802, USA





*

Author to whom correspondence should be addressed.



Academic Editor: Derek J. McPhee

Abstract Gastric cancer is one of the most common malignant cancers, with high death rates, poor prognosis and limited treatment methods. Cyclovirobuxine D CVB-D is the main active component of the traditional Chinese medicine Buxus microphylla. In the present study, we test the effects of CVB-D on gastric cancer cells and the underlying mechanisms of action. CVB-D reduced cell viability and colony formation ability of MGC-803 and MKN28 cells in a time- and concentration-dependent manner. Flow cytometry showed that cell cycle of CVB-D treated cells was arrested at the S-phase. CVB-D also induced apoptosis in MGC-803 and MKN28 cells, especially early stage apoptosis. Furthermore, mitochondria membrane potential Δψm was reduced and apoptosis-related proteins, cleaved Caspase-3 and Bax-Bcl-2, were up-regulated in CVB-D-treated MGC-803 and MKN28 cells. Taken together, our studies found that CVB-D plays important roles in inhibition of gastric tumorigenesis via arresting cell cycle and inducing mitochondria-mediated apoptosis, suggesting the potential application of CVB-D in gastric cancer therapy. View Full-Text

Keywords: cyclovirobuxine D; cell cycle; gastric cancer; apoptosis; mitochondria cyclovirobuxine D; cell cycle; gastric cancer; apoptosis; mitochondria





Autor: Jie Wu 1,2,* , Zhujun Tan 3, Jian Chen 4 and Cheng Dong 1,5

Fuente: http://mdpi.com/



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