DNA Catalysis: The Chemical Repertoire of DNAzymesReportar como inadecuado




DNA Catalysis: The Chemical Repertoire of DNAzymes - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, CH-3012 Bern, Switzerland



Present address: Institut Pasteur, Chemistry and Structural Biology Department, Laboratory for Bioorganic Chemistry of Nucleic Acids, 28 rue du Dr Roux, 75724 Paris cedex 15, France.





Academic Editor: Hui Wei

Abstract Deoxyribozymes or DNAzymes are single-stranded catalytic DNA molecules that are obtained by combinatorial in vitro selection methods. Initially conceived to function as gene silencing agents, the scope of DNAzymes has rapidly expanded into diverse fields, including biosensing, diagnostics, logic gate operations, and the development of novel synthetic and biological tools. In this review, an overview of all the different chemical reactions catalyzed by DNAzymes is given with an emphasis on RNA cleavage and the use of non-nucleosidic substrates. The use of modified nucleoside triphosphates dN*TPs to expand the chemical space to be explored in selection experiments and ultimately to generate DNAzymes with an expanded chemical repertoire is also highlighted. View Full-Text

Keywords: SELEX; DNAzymes; chemically modified nucleic acids; functional nucleic acids; biosensors; therapeutic nucleic acids SELEX; DNAzymes; chemically modified nucleic acids; functional nucleic acids; biosensors; therapeutic nucleic acids





Autor: Marcel Hollenstein †

Fuente: http://mdpi.com/



DESCARGAR PDF




Documentos relacionados