Isoeugenin, a Novel Nitric Oxide Synthase Inhibitor Isolated from the Rhizomes of Imperata cylindricaReport as inadecuate




Isoeugenin, a Novel Nitric Oxide Synthase Inhibitor Isolated from the Rhizomes of Imperata cylindrica - Download this document for free, or read online. Document in PDF available to download.

1

Department of Pharmacology, College of Korean Medicine, Sangji University, Wonju 220-702, Korea

2

Department of Agro-Industrial Technology, Faculty of Agriculture, Lambung Mangkurat University, Indonesia 70712, Indonesia

3

Department of Forest Science, College of Life Science and Natural Resources, Sangji University, Wonju 220-702, Korea

4

Department of Pharmaceutical Engineering, College of Health Science, Sangji University, Wonju 220-702, Korea





*

Author to whom correspondence should be addressed.



Academic Editors: Derek J. McPhee and John A. Beutler

Abstract Phytochemical studies on the constituents of the rhizomes of Imperata cylindrica Gramineae were performed using high-performance liquid chromatography HPLC. We also aimed to search for any biologically active substance capable of inhibiting nitric oxide NO formation in lipopolysaccharide LPS-activated macrophage 264.7 cells, by testing four compounds isolated from this plant. Four compounds, including a new chromone, isoeugenin, along with ferulic acid, p-coumaric acid, and caffeic acid were isolated and identified by NMR spectroscopy. The structure of isoeugenin was determined as 7-hydroxy-5-methoxy-2-methylchromone by the 2D-NMR technique. Among the four compounds, isoeugenin has the lowest IC50 value on the inhibition of NO production in LPS-activated macrophage RAW264.7 cells IC50, 9.33 μg-mL. In addition, isoeugenin significantly suppressed the LPS-induced expressions of inducible nitric oxide synthase iNOS, cyclooxygenase-2 COX-2, and proinflammatory cytokines mRNA levels. Taken together, these results suggest that the anti-inflammatory activity of isoeugenin is associated with the down-regulation of iNOS, COX-2, and pro-inflammatory cytokines in RAW264.7 cells. Accordingly, our results suggest that the new chromone isoegenin should be considered a potential treatment for inflammatory disease. View Full-Text

Keywords: Imperata cylindrica; isoeugenin; RAW264.7 macrophages; NO; HPLC Imperata cylindrica; isoeugenin; RAW264.7 macrophages; NO; HPLC





Author: Hyo-Jin An 1, Agung Nugroho 2, Byong-Min Song 3 and Hee-Juhn Park 4,*

Source: http://mdpi.com/



DOWNLOAD PDF




Related documents