Analysis of Chemical Constituents in Wuzi-Yanzong-Wan by UPLC-ESI-LTQ-Orbitrap-MSReport as inadecuate

Analysis of Chemical Constituents in Wuzi-Yanzong-Wan by UPLC-ESI-LTQ-Orbitrap-MS - Download this document for free, or read online. Document in PDF available to download.


Beijing Key Lab of Chinese Materia Medica Quality Evaluation, School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China


Beijing Tong Ren Tang Group Co., Ltd., Beijing 100051, China


Author to whom correspondence should be addressed.

Academic Editor: Christopher W. K. Lam

Abstract Wuzi-Yanzong-Wan WZYZW, a classical traditional Chinese medicine TCM prescription containing Fructus Lych, Semen Cuscutae fried, Fructus Rubi, Fructus Schisandrae chinensis steamed and Semen Plantaginis fried with salt, is widely used to treat impotence, sterility, spermatorrhea, premature ejaculation, lumbago and post-micturation dribble. However, the chemical profile of WZYZW has not been established yet. In this work, a rapid and sensitive method for systematically screening and identifying the chemical constituents of WZYZW in both positive and negative ion modes using Ultra-Performance LC coupled with ESI-linear ion trap-Orbitrap tandem mass spectrometry UPLC-ESI-LTQ-Orbitrap-MS has been developed. Based on the chromatographic and spectrometric data, and referring to the literature, we could tentatively identify 106 compounds, including organic acids, flavonoids, phenylpropanoids, alkaloids and terpenoids. Fourteen ingredients from Fructus Lych were identified, while 10 ingredients were from Semen Cuscutae fried, 33 ingredients were from Fructus Rubi, 37 ingredients were from Fructus Schisandrae chinensis steamed, and 20 ingredients were from Semen Plantaginis fried with salt. The results may provide essential data for further quality control, pharmacological research and clinical evaluation of WZYZW. Furthermore, this study indicates the developed approach based on UPLC-ESI-LTQ-Orbitrap-MS is suitable for characterizing the chemical profiles of TCM prescriptions. This is the first report to provide a comprehensive analysis of the chemical constituents of WZYZW. View Full-Text

Keywords: Wuzi-Yanzong-Wan; UPLC-ESI-LTQ-Orbitrap-MS; constituents; identification Wuzi-Yanzong-Wan; UPLC-ESI-LTQ-Orbitrap-MS; constituents; identification

Author: Dixin Zou 1, Jinfeng Wang 1, Bo Zhang 1, Suhua Xie 2, Qing Wang 1, Kexin Xu 1 and Ruichao Lin 1,*



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