Accurate Mass GC-LC-Quadrupole Time of Flight Mass Spectrometry Analysis of Fatty Acids and Triacylglycerols of Spicy Fruits from the Apiaceae FamilyReportar como inadecuado




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1

Department of Chemistry, Texas Southern University, Houston, TX 77004, USA

2

Agilent Technologies, Inc., 3750 Brookside Parkway, Suite 100, Alpharetta, GA 30022, USA





*

Author to whom correspondence should be addressed.



Academic Editor: Derek J. McPhee

Abstract The triacylglycerol TAG structure and the regio-stereospecific distribution of fatty acids FA of seed oils from most of the Apiaceae family are not well documented. The TAG structure ultimately determines the final physical properties of the oils and the position of FAs in the TAG molecule affects the digestion; absorption and metabolism; and physical and technological properties of TAGs. Fixed oils from the fruits of dill Anethum graveolens, caraway Carum carvi, cumin Cuminum cyminum, coriander Coriandrum sativum, anise Pimpinella anisum, carrot Daucus carota, celery Apium graveolens, fennel Foeniculum vulgare, and Khella Ammi visnaga, all from the Apiaceae family, were extracted at room temperature in chloroform-methanol 2:1 v-v using percolators. Crude lipids were fractionated by solid phase extraction to separate neutral triacylglycerols TAGs from other lipids components. Neutral TAGs were subjected to transesterification process to convert them to their corresponding fatty acids methyl esters FAMES using 1% boron trifluoride BF3 in methanol. FAMES were analyzed by gas chromatography-quadrupole time of flight GC-QTOF mass spectrometry. Triglycerides were analyzed using high performance liquid chromatography-quadrupole time of flight LC-QTOF mass spectrometry. Petroselinic acid was the major fatty acid in all samples ranging from 57% of the total fatty acids in caraway up to 82% in fennel. All samples contained palmitic 16:0, palmitoleic C16:1n-9, stearic C18:0, petroselinic C18:1n-12, linoleic C18:2n-6, linolinic 18:3n-3, and arachidic C20:0 acids. TAG were analyzed using LC-QTOF for accurate mass identification and mass spectrometry-mass spectrometry MS-MS techniques for regiospesific elucidation of the identified TAGs. Five major TAGs were detected in all samples but with different relative concentrations in all of the tested samples. Several other TAGs were detected as minor components and were present in some samples and absence in the others. Regiospecific analysis showed a non-random fatty acids distribution. Petroselinic acid was predominantly located at the sn-1 and sn-3 positions. View Full-Text

Keywords: seed oil; petroselinic acid; regiospecific; lipids; QTOF seed oil; petroselinic acid; regiospecific; lipids; QTOF





Autor: Thao Nguyen 1, Mario Aparicio 2 and Mahmoud A. Saleh 1,*

Fuente: http://mdpi.com/



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