Enrichment and Purification of Polyphenol Extract from Sphallerocarpus gracilis Stems and Leaves and in Vitro Evaluation of DNA Damage-Protective Activity and Inhibitory Effects of α-Amylase and α-GlucosidaseReportar como inadecuado




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1

College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710062, China

2

College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China

3

National HACCP Center, Chinese Academy of Inspection and Quarantine, Beijing 100123, China





*

Authors to whom correspondence should be addressed.



Academic Editors: Isabel C. F. R. Ferreira and Nancy D. Turner

Abstract An efficient preparative separation method for Sphallerocarpus gracilis stems and leaves polyphenols SGslP was established in this study. An X-5 macroporous adsorption resin was selected for the purification of the SGslP, and the polyphenol content of the purified SGslP PSGslP was increased 5.11-fold from 8.29% to 42.38% after one treatment run. The chemical composition of the PSGslP was analyzed by HPLC-MS-MS, and the predominant compounds were found to be luteolin-7-glucoside, acacetin-7-acetyglycoside and its isomers. In addition, the PSGslP was evaluated in vitro to determine the DNA damage-protective activity and inhibitory effects of α-amylase and α-glucosidase. The results indicated that the PSGslP exhibited significant protective activities against both ROO• and •OH radical-induced DNA damage. Moreover, the PSGslP exerted a dose-dependent inhibition effect on α-glucosidase but no inhibitory effect on α-amylase. These findings indicate that the Sphallerocarpus gracilis stems and leaves are good natural sources of antioxidants and are potent inhibitors of α-glucosidase activity and are potential anti-diabetic inhibitor. View Full-Text

Keywords: SGslP; purification; phenolic composition; oxidative DNA damage; enzyme inhibition SGslP; purification; phenolic composition; oxidative DNA damage; enzyme inhibition





Autor: Tingting Ma 1,2, Xiangyu Sun 2, Chengrui Tian 1,* , Jiyang Luo 3, Cuiping Zheng 1 and Jicheng Zhan 2,*

Fuente: http://mdpi.com/



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