Oral Administration of Achyranthis radix Extract Prevents TMA-induced Allergic Contact Dermatitis by Regulating Th2 Cytokine and Chemokine Production in VivoReportar como inadecuado




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1

Research Group of Nutraceuticals for Metabolic Syndrome, Korea Food Research Institute, Seongnam 13539, Korea

2

Food Biotechnology Program, Korea University of Science and Technology, Daejeon 34113, Korea

3

Research Group of Metabolic Mechanism, Korea Food Research Institute, Seongnam 13539, Korea



These authors contributed equally to this work.





*

Author to whom correspondence should be addressed.



Academic Editor: Maurizio Battino

Abstract Allergic contact dermatitis ACD remains a major skin disease in many countries, necessitating the discovery of novel and effective anti-ACD agents. In this study, we investigated the preventive effects of Achyranthis radix extract AcRE on trimellitic anhydride TMA-induced dermatitis and the potential mechanism of action involved. Oral administration of AcRE and prednisolone PS significantly suppressed TMA-induced increases in ear and epidermal thickness, and IgE expression. In addition, abnormal expression of IL-1β and TNF-α protein and mRNA was also significantly attenuated by oral administration of AcRE. Treatment with AcRE also significantly suppressed TMA-induced IL-4 and IL-13 cytokines and mRNA expression in vivo. Moreover, AcRE strongly suppressed TMA-induced IL-4 and IL-5 production in draining lymph nodes, as well as OVA-induced IL-4 and IL-5 expression in primary cultured splenocytes. Interestingly, AcRE suppressed IL-4-induced STAT6 phosphorylation in both primary cultured splenocytes and HaCaT cells, and TMA-induced GATA3 mRNA expression ex vivo. AcRE also suppressed TMA-mediated CCL11 and IL-4-induced CCL26 mRNA expression and infiltration of CCR3 positive cells. The major compounds from AcRE were identified as gentisic acid 0.64 ± 0.2 μg-g dry weight of AcRE, protocatechuic acid 2.69 ± 0.1 μg-g dry weight of AcRE, 4-hydroxybenzoic acid 5.59 ± 0.3 μg-g dry weight of AcRE, caffeic acid 4.21 ± 0.1 μg-g dry weight of AcRE, and ferulic acid 14.78 ± 0.4 ± 0.3 μg-g dry weight of AcRE. Taken together, these results suggest that AcRE has potential for development as an agent to prevent and treat allergic contact dermatitis. View Full-Text

Keywords: Achyranthis radix; allergic contact dermatitis; Th2 cytokines; chemokines; STAT6 Achyranthis radix; allergic contact dermatitis; Th2 cytokines; chemokines; STAT6





Autor: Sung Keun Jung 1,2,†, Dae Woon Choi 1,2,†, Da-Ae Kwon 1, Min Jung Kim 3, Ki Seung Seong 1 and Dong-Hwa Shon 1,2,*

Fuente: http://mdpi.com/



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