1-Deoxynojirimycin Alleviates Insulin Resistance via Activation of Insulin Signaling PI3K-AKT Pathway in Skeletal Muscle of db-db MiceReport as inadecuate




1-Deoxynojirimycin Alleviates Insulin Resistance via Activation of Insulin Signaling PI3K-AKT Pathway in Skeletal Muscle of db-db Mice - Download this document for free, or read online. Document in PDF available to download.

1

College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China

2

Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing 210023, China



These authors contributed equally to this work.





*

Author to whom correspondence should be addressed.



Academic Editor: Derek J. McPhee

Abstract 1-Deoxynojirimycin DNJ is widely used for the treatment of diabetes mellitus as an inhibitor of intestinal α-glucosidase. However, there are few reports about its effect on insulin sensitivity improvement. The aim of the present study was to investigate whether DNJ decreased hyperglycemia by improving insulin sensitivity. An economical method was established to prepare large amounts of DNJ. Then, db-db mice were treated with DNJ intravenously 20, 40 and 80 mg·kg−1·day−1 for four weeks. Blood glucose and biochemical analyses were conducted to evaluate the therapeutic effects on hyperglycemia and the related molecular mechanisms in skeletal muscle were explored. DNJ significantly reduced body weight, blood glucose and serum insulin levels. DNJ treatment also improved glucose tolerance and insulin tolerance. Moreover, although expressions of total protein kinase B AKT, phosphatidylinositol 3 kinase PI3K, insulin receptor beta IR-β, insulin receptor substrate-1 IRS1 and glucose transporter 4 GLUT4 in skeletal muscle were not affected, GLUT4 translocation and phosphorylation of Ser473-AKT, p85-PI3K, Tyr1361-IR-β and Tyr612-IRS1 were significantly increased by DNJ treatment. These results indicate that DNJ significantly improved insulin sensitivity via activating insulin signaling PI3K-AKT pathway in skeletal muscle of db-db mice. View Full-Text

Keywords: mulberry leaves; 1-deoxynojirimycin; db-db mice; insulin resistance; insulin signaling pathway; skeletal muscle; PI3K-AKT; GLUT4 translocation mulberry leaves; 1-deoxynojirimycin; db-db mice; insulin resistance; insulin signaling pathway; skeletal muscle; PI3K-AKT; GLUT4 translocation





Author: Qingpu Liu 1,†, Xuan Li 1,†, Cunyu Li 1,2, Yunfeng Zheng 1,2 and Guoping Peng 1,2,*

Source: http://mdpi.com/



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