Seapolynol Extracted from Ecklonia cava Inhibits Adipocyte Differentiation in Vitro and Decreases Fat Accumulation in VivoReportar como inadecuado




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1

Department of Food Science and Biotechnology, CHA University, Seongnam, Gyeonggi 463-400, Korea

2

Department of Food Science and Technology, Seoul Womens University, Hwarang, Nowon, Seoul 139-774, Korea

3

Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, Korea



These authors contributed equally to this work.





*

Author to whom correspondence should be addressed.



Academic Editor: Derek J. McPhee

Abstract Seapolynol SN is a polyphenol mixture derived from Ecklonia cava. We evaluated the effects of SN on lipid accumulation in adipocytes, zebrafish, and mice. SN effectively inhibited lipid accumulation in three experimental models by suppressing adipogenic factors. Triglyceride synthetic enzymes such as diacylglycerol acyltransferase 1 DGAT1 and GPAT3 were also downregulated by SN. This SN-induced inhibition of adipogenic factors was shown to be due to the regulatory effect of SN on early adipogenic factors; SN downregulated the expression of Krueppel-like factor 4 KLF4, KLF5, CCAAT-enhancer-binding protein β C-EBPβ, C-EBPδ, and Protein C-ets-2 ETS2, while KLF2, an anti-early adipogenic factor, was upregulated by SN. SN-mediated inhibition in early adipogenesis was closely correlated with the inhibition of mitotic clonal expansion via cell cycle arrest. SN inhibited cell cycle progression by suppressing cell cycle regulators, such as cyclin A, cyclinD, and pRb but increased p27, a cell cycle inhibitor. In a mouse study, SN effectively reduced body weight and plasma lipid increases induced by a high-fat diet; triglycerides, total cholesterol, and low-density lipoprotein LDL levels were markedly reduced by SN. Moreover, SN remarkably improved high-fat-diet-induced hepatic lipid accumulation. Furthermore, SN activated AMP-activated protein kinase-α AMPKα, an energy sensor, to suppress acetyl-coA carboxylase ACC, inhibiting lipid synthesis. Our study suggests that SN may be an edible agent that can play a positive role in prevention of metabolic disorders. View Full-Text

Keywords: seapolynol; adipogenesis; 3T3-L1; zebrafish; ICR mouse seapolynol; adipogenesis; 3T3-L1; zebrafish; ICR mouse





Autor: Hui-Jeon Jeon 1,†, Hyeon-Son Choi 2,†, Yeon-Joo Lee 1, Ji-Hyun Hwang 1, Ok-Hwan Lee 3, Min-Jung Seo 1, Kui-Jin Kim 1 and Boo-Yong Lee 1,*

Fuente: http://mdpi.com/



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