Safety Evaluation, in Vitro and in Vivo Antioxidant Activity of the Flavonoid-Rich Extract from Maydis stigmaReport as inadecuate




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1

College of Chemical and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China

2

College of pharmacy, Zhengzhou University, Zhengzhou 450001, China

3

Department of Mathematics, School of Public Health, Jilin Medical College, Jilin 132013, China





*

Author to whom correspondence should be addressed.



Academic Editor: Christopher W. K. Lam

Abstract This study aimed to assess the acute toxicity and safety of flavonoid-rich extract from Maydis stigma FMS in mice. The in vitro antioxidant activity of FMS was determined by 1,1-diphenyl-2-picrylhydrazyl DPPH and 2,2′-azinobis-3-ethyl-benzthiazoline-6-sulphonate ABTS scavenging assays. Furthermore, the in vivo antioxidant of FMS against ethanol-induced oxidative damage in mice was determined by analysis of the serum total superoxide dismutase T-SOD activity, malondialdehyde MDA content, liver tissue glutathione GSH content, and protein carbonyl PC content in liver tissue. The oral administration of FMS at doses of 30 g-kg did not cause death in mice, and there were no significant biologically adverse effects in mice. These results indicated that the median lethal dose LD50 is higher than this dose. The IC50 values of FMS for the DPPH and ABTS scavenging activity were 50.73 and 0.23 mg-mL, respectively. Meanwhile, FMS could significantly enhance T-SOD activity, reduce MDA content in the serum, increase GSH content, and decrease PC content in the liver tissue at the tested doses 25, 50, 100, 200 mg-kg·day. These results indicate that FMS can be generally regarded as safe and used potentially as a bioactive source of natural antioxidants. View Full-Text

Keywords: flavonoids; Maydis stigma; acute toxicity; antioxidant activity flavonoids; Maydis stigma; acute toxicity; antioxidant activity





Author: Ke-Zheng Peng 1,2, Xiudong Yang 1, Hong-Li Zhou 1,* and Shu-Xia Pan 3

Source: http://mdpi.com/



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