Multi-Optimization of Ultrasonic-Assisted Enzymatic Extraction of Atratylodes macrocephala Polysaccharides and Antioxidants Using Response Surface Methodology and Desirability Function ApproachReportar como inadecuado




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1

Development and Research Center of Official Silkworm Resources, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou 310007, China

2

Key Laboratory of Research and Development of Chinese Medicine of Zhejiang Province, Hangzhou 310007, China

3

College of Pharmacy, Hunan Chinese Medical University, Changsha 410208, China

4

College of Life Science, Zhejiang Chinese Medical University, Hangzhou 310015, China



These authors contributed equally to this work.





*

Author to whom correspondence should be addressed.



Academic Editor: Derek J. McPhee

Abstract Rhizoma Atractylodes macrocephala polysaccharides RAMP have been reported to have a variety of important biological activities. In this study, an ultrasonic-assisted enzymatic extraction UAEE was employed to obtain the highest extraction yield and strongest antioxidant activity of RAMP and optimized by a multi-response optimization process. A three-level four-factor Box-Behnken design BBD was performed as response surface methodology RSM with desirability function DF to attain the optimal extraction parameters. The DPPH scavenging percentage was used to represent the antioxidant ability of RAMP. The maximum D value 0.328, along with the maximum yield 59.92% and DPPH scavenging percentage 13.28% were achieved at 90.54 min, 57.99 °C, 1.95% cellulase and 225.29 W. These values were further validated and found to be in good agreement with the predicted values. Compared to the other extraction methods, both the yield and scavenging percentage of RAMP obtained by UAEE was favorable and the method appeared to be time-saving and of high efficiency. These results demostrated that UAEE is an appropriate and effective extraction technique. Moreover, RSM with DF approach has been proved to be adequate for the design and optimization of the extraction parameters for RAMP. This work has a wide range of implications for the design and operation of polysaccharide extraction processes. View Full-Text

Keywords: Rhizoma Atractylodes macrocephala polysaccharides; ultrasonic-assisted enzymatic extraction; response surface methodology; desirability function approach; antioxidant activity; multi-optimization Rhizoma Atractylodes macrocephala polysaccharides; ultrasonic-assisted enzymatic extraction; response surface methodology; desirability function approach; antioxidant activity; multi-optimization





Autor: Jin-Bao Pu 1,2,†, Bo-Hou Xia 3,†, Yi-Juan Hu 1,2, Hong-Jian Zhang 1,2, Jing Chen 4, Jie Zhou 1,2, Wei-Qing Liang 1,2 and Pan Xu 1,2,*

Fuente: http://mdpi.com/



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