Phenolic Profiling of Duchesnea indica Combining Macroporous Resin Chromatography MRC with HPLC-ESI-MS-MS and ESI-IT-MSReport as inadecuate




Phenolic Profiling of Duchesnea indica Combining Macroporous Resin Chromatography MRC with HPLC-ESI-MS-MS and ESI-IT-MS - Download this document for free, or read online. Document in PDF available to download.

1

Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China

2

State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Macau, China

3

Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan 430074, China



These authors contributed equally to this work.





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Author to whom correspondence should be addressed.



Academic Editors: Isabel Ferreira and Derek J. McPhee

Abstract Duchesnea indica D. indica is an important traditional Chinese medicine, and has long been clinically used to treat cancer in Asian countries. It has been described previously as a rich source of phenolic compounds with a broad array of diversified structures, which are the major active ingredients. However, an accurate and complete phenolic profiling has not been determined yet. In the present work, the total phenolic compounds in crude extracts from D. indica were enriched and fractionated over a macroporous resin column, then identified by HPLC-ESI-MS-MS and ESI-IT-MS ion trap MS. A total of 27 phenolic compounds were identified in D. indica, of which 21 compounds were identified for the first time. These 27 phenolic compounds encompassing four phenolic groups, including ellagitannins, ellagic acid and ellagic acid glycosides, hydroxybenzoic acid and hydroxycinnamic acid derivatives, and flavonols, were then successfully quantified using peak areas against those of the corresponding standards with good linearity R2 > 0.998 in the range of the tested concentrations. As a result, the contents of individual phenolic compounds varied from 6.69 mg per 100 g dry weight DW for ellagic acid to 71.36 mg per 100 g DW for brevifolin carboxylate. Not only did this study provide the first phenolic profiling of D. indica, but both the qualitative identification and the subsequent quantitative analysis of 27 phenolic compounds from D. indica should provide a good basis for future exploration of this valuable medicinal plant. View Full-Text

Keywords: Duchesnea indica; phenolic profiling; HPLC; mass spectrometry Duchesnea indica; phenolic profiling; HPLC; mass spectrometry





Author: Mingzhi Zhu 1,2,†, Xia Dong 1,† and Mingquan Guo 1,3,*

Source: http://mdpi.com/



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