Short-Term and Sub-Chronic Dietary Exposure to Aspalathin-Enriched Green Rooibos Aspalathus linearis Extract Affects Rat Liver Function and Antioxidant StatusReport as inadecuate




Short-Term and Sub-Chronic Dietary Exposure to Aspalathin-Enriched Green Rooibos Aspalathus linearis Extract Affects Rat Liver Function and Antioxidant Status - Download this document for free, or read online. Document in PDF available to download.

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Department of Food Science, Stellenbosch University, Private Bag X1, Matieland Stellenbosch 7602, South Africa

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Post-Harvest and Wine Technology Division, Agricultural Research Council ARC, Infruitec-Nietvoorbij, Private Bag X5026, Stellenbosch 7599, South Africa

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Institute of Biomedical and Microbial Biotechnology, Cape Peninsula University of Technology, P. O. Box 1906, Bellville 7535, South Africa

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Department of Biochemistry, Stellenbosch University, Private Bag X1, Matieland Stellenbosch 7602, South Africa



Current address: Food and Allergy Consulting and Testing Services, 18 Uplands Road, Cape Town 7435, South Africa





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Author to whom correspondence should be addressed.



Academic Editor: David D. Kitts

Abstract An aspalathin-enriched green rooibos Aspalathus linearis extract GRE was fed to male Fischer rats in two independent studies for 28 and 90 days. The average dietary total polyphenol TP intake was 756 and 627 mg Gallic acid equivalents GAE-kg body weight bw-day over 28 and 90 days, respectively, equaling human equivalent doses HEDs of 123 and 102 GAE mg-kg bw-day. Aspalathin intake of 295 mg-kg bw-day represents a HED of 48 mg-kg bw-day 90 day study. Consumption of GRE increased feed intake significantly p < 0.05 compared to the control after 90 days, but no effect on body and organ weight parameters was observed. GRE significantly p < 0.05 reduced serum total cholesterol and iron levels, whilst significantly p < 0.05 increasing alkaline phosphatase enzyme activity after 90 days. Endogenous antioxidant enzyme activity in the liver, i.e., catalase and superoxide dismutase activity, was not adversely affected. Glutathione reductase activity significantly p < 0.05 increased after 28 days, while glutathione GSH content was decreased after 90 days, suggesting an altered glutathione redox cycle. Quantitative Real Time polymerase chain reaction PCR analysis showed altered expression of certain antioxidant defense and oxidative stress related genes, indicative, among others, of an underlying oxidative stress related to changes in the GSH redox pathway and possible biliary dysfunction. View Full-Text

Keywords: herbal tea; biochemical parameters; serum iron; endogenous antioxidant enzymes; gene expression herbal tea; biochemical parameters; serum iron; endogenous antioxidant enzymes; gene expression





Author: Johanna Debora van der Merwe 1,†, Dalene de Beer 2, Elizabeth Joubert 1,2 and Wentzel C. A. Gelderblom 3,4,*

Source: http://mdpi.com/



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