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1

Department of Microbiology and Medical Laboratory Immunology, Medical University of Lodz, 92-231 Lodz, Pomorska 251, Poland

2

Department of Environmental Biology, Medical University of Lodz, 90-752 Lodz, Żeligowskiego 7-9, Poland

3

Department of General Dentistry, Medical University of Lodz, 92-231 Lodz, Pomorska 251, Poland





*

Author to whom correspondence should be addressed.



Academic Editor: Derek J. McPhee

Abstract Enterococci are able to survive endodontic procedures and contribute to the failure of endodontic therapy. Thus, it is essential to identify novel ways of eradicating them from infected root canals. One such approach may be the use of antimicrobials such as plant essential oils. Enterococcal strains were isolated from endodontically treated teeth by standard microbiological methods. Susceptibility to antibiotics was evaluated by the disc-diffusion method. The minimal inhibitory concentration MIC of geranium essential oil was investigated by microdilution in 96-well microplates in Mueller Hinton Broth II. Biofilm eradication concentrations were checked in dentin tests. Geranium essential oil inhibited enterococcal strains at concentrations ranging from 1.8–4.5 mg-mL. No correlation was shown between resistance to antibiotics and the MICs of the test antimicrobials. The MICs of the test oil were lower than those found to show cytotoxic effects on the HMEC-1 cell line. Geranium essential oil eradicated enterococcal biofilm at concentrations of 150 mg-mL. Geranium essential oil inhibits the growth of endodontic enterococcal species at lower concentrations than those required to reach IC50 against the HMEC-1 cell line, and is effective against bacteria protected in biofilm at higher concentrations. In addition, bacteria do not develop resistance to essential oils. Hence, geranium essential oil represents a possible alternative to other antimicrobials during endodontic procedures. View Full-Text

Keywords: enterococci; endodontic treatment; geranium essential oil enterococci; endodontic treatment; geranium essential oil





Autor: Monika E. Łysakowska 1,* , Monika Sienkiewicz 2, Katarzyna Banaszek 3 and Jerzy Sokołowski 3

Fuente: http://mdpi.com/



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