Preparative Isolation of Two Prenylated Biflavonoids from the Roots and Rhizomes of Sinopodophyllum emodi by Sephadex LH-20 Column and High-Speed Counter-Current ChromatographyReport as inadecuate




Preparative Isolation of Two Prenylated Biflavonoids from the Roots and Rhizomes of Sinopodophyllum emodi by Sephadex LH-20 Column and High-Speed Counter-Current Chromatography - Download this document for free, or read online. Document in PDF available to download.

1

Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment and Chinese Medicine Development of Henan Province, Henan University of Traditional Chinese Medicine, Zhengzhou 450046, Henan, China

2

School of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou 450046, Henan, China

3

Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, Liaoning, China

4

School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, Liaoning, China

5

Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, Jilin, China





*

Authors to whom correspondence should be addressed.



Academic Editor: Derek J. McPhee

Abstract Two prenylated biflavonoids, podoverines B–C, were isolated from the dried roots and rhizomes of Sinopodophyllum emodi using a Sephadex LH-20 column SLHC and high-speed counter-current chromatography HSCCC. The 95% ethanol extract was partitioned with ethyl acetate in water. Target compounds from the ethyl acetate fraction were further enriched and purified by the combined application of SLHC and HSCCC. n-Hexane–ethyl acetate–methanol–water 3.5:5:3.5:5, v-v was chosen as the two phase solvent system. The flow rate of mobile phase was optimized at 2.0 mL·min−1. Finally, under optimized conditions, 13.8 mg of podoverine B and 16.2 mg of podoverine C were obtained from 200 mg of the enriched sample. The purities of podoverines B and C were 98.62% and 99.05%, respectively, as determined by HPLC. For the first time, podoverins B and C were found in the genus Sinopodophyllum. Their structures were determined by spectroscopic methods HR-ESI-MS, 1H-NMR, 13C-NMR, HSQC, HMBC. Their absolute configurations were elucidated by comparison of their experimental and calculated ECD spectra. The cytotoxic activities were evaluated against MCF-7 and HepG2 cell lines. The separation procedures proved to be practical and economical, especially for trace prenylated biflavonoids from traditional Chinese medicine. View Full-Text

Keywords: Sinopodophyllum emodi; prenylated biflavonoid; Sephadex LH-20 column chromatography; high-speed counter-current chromatography; cytotoxic activity Sinopodophyllum emodi; prenylated biflavonoid; Sephadex LH-20 column chromatography; high-speed counter-current chromatography; cytotoxic activity





Author: Yan-Jun Sun 1,2,* , Li-Xin Pei 1,2, Kai-Bo Wang 3,4, Yin-Shi Sun 5,* , Jun-Min Wang 1,2, Yan-Li Zhang 1,2, Mei-Ling Gao 1,2 and Bao-Yu Ji 1,2

Source: http://mdpi.com/



DOWNLOAD PDF




Related documents