Isolation of Terpenoids from the Stem of Ficus aurantiaca Griff and their Effects on Reactive Oxygen Species Production and Chemotactic Activity of NeutrophilsReportar como inadecuado




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Drug and Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia UKM, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, Malaysia





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Academic Editor: Derek J. McPhee

Abstract Three new triterpenoids; namely 28,28,30-trihydroxylupeol 1; 3,21,21,26-tetrahydroxy-lanostanoic acid 2 and dehydroxybetulinic acid 3 and seven known compounds; i.e., taraxerone 4; taraxerol 5; ethyl palmitate 6; herniarin 7; stigmasterol 8; ursolic acid 9 and acetyl ursolic acid 10 were isolated from the stem of Ficus aurantiaca Griff. The structures of the compounds were established by spectroscopic techniques. The compounds were evaluated for their inhibitory effects on polymorphonuclear leukocyte PMN chemotaxis by using the Boyden chamber technique and on human whole blood and neutrophil reactive oxygen species ROS production by using a luminol-based chemiluminescence assay. Among the compounds tested, compounds 1–4, 6 and 9 exhibited strong inhibition of PMN migration towards the chemoattractant N-formyl-methionyl-leucyl-phenylalanine fMLP with IC50 values of 6.8; 2.8; 2.5; 4.1; 3.7 and 3.6 μM, respectively, comparable to that of the positive control ibuprofen 6.7 μM. Compounds 2–4, 6, 7 and 9 exhibited strong inhibition of ROS production of PMNs with IC50 values of 0.9; 0.9; 1.3; 1.1; 0.5 and 0.8 μM, respectively, which were lower than that of aspirin 9.4 μM. The bioactive compounds might be potential lead molecules for the development of new immunomodulatory agents to modulate the innate immune response of phagocytes. View Full-Text

Keywords: Ficus aurantiaca Griff; terpenoids; immunomodulatory; neutrophils; chemotaxis; reactive oxygen species Ficus aurantiaca Griff; terpenoids; immunomodulatory; neutrophils; chemotaxis; reactive oxygen species





Autor: Shukranul Mawa, Ibrahim Jantan and Khairana Husain *

Fuente: http://mdpi.com/



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