A New Indirect Spectrofluorimetric Method for Determination of Ascorbic Acid with 2,4,6-Tripyridyl-S-Triazine in Pharmaceutical SamplesReportar como inadecuado




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1

Department of Chemistry, Faculty of Science, University of Sarajevo, Zmaja od Bosne 33-35, Sarajevo 71 000, Bosnia and Herzegovina

2

Faculty of Chemistry and Technology, University of Split, Teslina 10, Split 21 000, Croatia



These authors contributed equally to this work.





*

Author to whom correspondence should be addressed.



Academic Editor: Derek J. McPhee

Abstract Ascorbic acid AA is a water-soluble vitamin which shows no fluorescence. However, in reaction with ironIII, AA is oxidised to dehydroascorbic acid and ironIII is reduced to ironII which forms a complex with 2,4,6-tripyridyl-S-triazine TPTZ in buffered medium. The relative fluorescence intensity of the resulting FeTPTZ22+ complex can be measured at excitation and emission wavelengths of 393 and 790 nm, respectively. Based on this data, a new indirect spectrofluorimetric method for the determination of AA in pharmaceutical samples was proposed. Influence of the reaction conditions, such as acidity of acetic buffer, concentration of TPTZ and ironIII, reaction time and instrumental parameters were investigated in detail. The linear range was from 5.4 × 10−4 to 5.4 × 10−6 mol·L−1 R = 0.9971. The LOD was 7.7 × 10−7 mol·L−1 and LOQ was 2.3 × 10−4 mol·L−1. Fourteen pharmaceutical samples containing various amounts of AA were analysed. Influences of potential interfering substances were also examined. Analysis of commercial pharmaceutical formulations showed good correlation with the nominal values given by the manufacturers and with the results obtained by a titration method. The proposed method can be applied in routine quality control in the pharmaceutical industry due to its sensitivity, simplicity, selectivity and low cost. View Full-Text

Keywords: spectrofluorimetry; ascorbic acid; 2,4,6-tripyridyl-S-triazine spectrofluorimetry; ascorbic acid; 2,4,6-tripyridyl-S-triazine





Autor: Lejla Klepo 1,†, Amira Copra-Janicijevic 1,†,* and Lea Kukoc-Modun 2

Fuente: http://mdpi.com/



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