Screening of Potential Xanthine Oxidase Inhibitors in Gnaphalium hypoleucum DC. by Immobilized Metal Affinity Chromatography and Ultrafiltration-Ultra Performance Liquid Chromatography-Mass SpectrometryReportar como inadecuado


Screening of Potential Xanthine Oxidase Inhibitors in Gnaphalium hypoleucum DC. by Immobilized Metal Affinity Chromatography and Ultrafiltration-Ultra Performance Liquid Chromatography-Mass Spectrometry


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Key Laboratory of Research and Development of Chinese Medicine of Zhejiang Province, Zhejiang Academy of Traditional Chinese Medicine, 132 Tianmushan Road, Hangzhou 310007, China





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Abstract In this study, a new method based on immobilized metal affinity chromatography IMAC combined with ultrafiltration-ultra performance liquid chromatography-mass spectrometry UF-UPLC-MS was developed for discovering ligands for xanthine oxidase XO in Gnaphalium hypoleucum DC., a folk medicine used in China for the treatment of gout. By IMAC, the high flavonoid content of G. hypoleucum could be determined rapidly and efficiently. UF-UPLC-MS was used to select the bound xanthine oxidase ligands in the mixture and identify them. Finally, two flavonoids, luteolin-4′-O-glucoside and luteolin, were successfully screened and identified as the candidate XO inhibitors of G. hypoleucum. They were evaluated in vitro for XO inhibitory activity and their interaction mechanism was studied coupled with molecular simulations. The results were in favor of the hypothesis that the flavonoids of G. hypoleucum might be the active content for gout treatment by inhibiting XO. View Full-Text

Keywords: Gnaphalium hypoleucum DC.; XO inhibitor; immobilized metal affinity chromatography; luteolin-4′-O-glucoside; luteolin Gnaphalium hypoleucum DC.; XO inhibitor; immobilized metal affinity chromatography; luteolin-4′-O-glucoside; luteolin





Autor: Hong-Jian Zhang, Yi-Juan Hu, Pan Xu, Wei-Qing Liang, Jie Zhou, Pei-Gang Liu, Lin Cheng and Jin-Bao Pu *

Fuente: http://mdpi.com/



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