Digital Gene Expression Profiling to Explore Differentially Expressed Genes Associated with Terpenoid Biosynthesis during Fruit Development in Litsea cubebaReportar como inadecuado


Digital Gene Expression Profiling to Explore Differentially Expressed Genes Associated with Terpenoid Biosynthesis during Fruit Development in Litsea cubeba


Digital Gene Expression Profiling to Explore Differentially Expressed Genes Associated with Terpenoid Biosynthesis during Fruit Development in Litsea cubeba - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

1

State Key Laboratory of Forest Genetics and Tree Breeding, Chinese Academy of Forestry, No.1 Dong Xiaofu, Beijing 10086, China

2

Research Institute of Subtropical Forestry, Chinese Academy of Forestry, No.73 Daqiao Road, Hangzhou 311400, Zhejiang Province, China





*

Author to whom correspondence should be addressed.



Academic Editor: Derek J. McPhee

Abstract Mountain pepper Litsea cubeba Lour. Pers. Lauraceae is an important industrial crop as an ingredient in cosmetics, pesticides, food additives and potential biofuels. These properties are attributed to monoterpenes and sesquiterpenes. However, there is still no integrated model describing differentially expressed genes DEGs involved in terpenoid biosynthesis during the fruit development of L. cubeba. Here, we performed digital gene expression DGE using the Illumina NGS platform to evaluated changes in gene expression during fruit development in L. cubeba. DGE generated expression data for approximately 19354 genes. Fruit at 60 days after flowering DAF served as the control, and a total of 415, 1255, 449 and 811 up-regulated genes and 505, 1351, 1823 and 1850 down-regulated genes were identified at 75, 90, 105 and 135 DAF, respectively. Pathway analysis revealed 26 genes involved in terpenoid biosynthesis pathways. Three DEGs had continued increasing or declining trends during the fruit development. The quantitative real-time PCR qRT-PCR results of five differentially expressed genes were consistent with those obtained from Illumina sequencing. These results provide a comprehensive molecular biology background for research on fruit development, and information that should aid in metabolic engineering to increase the yields of L. cubeba essential oil. View Full-Text

Keywords: terpenoid biosynthesis; digital gene expression; differentially expressed genes; quantitative real-time PCR; Litsea cubeba Lour. Pers. terpenoid biosynthesis; digital gene expression; differentially expressed genes; quantitative real-time PCR; Litsea cubeba Lour. Pers.





Autor: Ming Gao 1,2, Liyuan Lin 1,2, Yicun Chen 1,2 and Yangdong Wang 1,2,*

Fuente: http://mdpi.com/



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