Cytoproliferative and Cytoprotective Effects of Striatisporolide A Isolated from Rhizomes of Athyrium multidentatum Doell. Ching on Human Umbilical Vein Endothelial CellsReportar como inadecuado


Cytoproliferative and Cytoprotective Effects of Striatisporolide A Isolated from Rhizomes of Athyrium multidentatum Doell. Ching on Human Umbilical Vein Endothelial Cells


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1

Department of Pharmacy, Weifang Medical University, Weifang 261053, China

2

Analysis and Test Center, Yianbian University, Yanji 133002, China

3

Plastic Surgery Institute of Weifang Medical University, Weifang 261053, China





*

Author to whom correspondence should be addressed.



Academic Editor: Marcello Iriti

Abstract Objectives: The aim of this study was to investigate the proliferative and protective effects of striatisporolide A SA obtained from the rhizomes of Athyrium multidentatum Doell. Ching on human umbilical vein endothelial cells HUVECs. Methods: Cell viability was measured by the MTT method. Cell apoptosis was determined by flow cytometry. Intracellular ROS was measured by the 2,7-dichlorodihydrofluorescein diacetate DCFH-DA fluorescent probe. Results: The viability rate in cells treated with 100 µM SA alone was increased to 128.72% ± 0.19% and showed a significant difference compared with the control group p < 0.05. Meanwhile, SA augmented the cell viabilities in H2O2-treated HUVECs, and the cell viability was enhanced to 56.94% ± 0.13% p < 0.01 when pre-incubated with 50 µM SA. The cell apoptosis rates were reduced to 2.17% ± 0.20% p < 0.05 and 3.1% ± 0.34% p < 0.01, respectively, after treatment with SA alone or SA-H2O2. SA inhibited the overproduction of reactive oxygen species ROS in HUVECs induced by H2O2 and the fluorescent intensity was abated to 9.47 ± 0.61 after pre-incubated with 100 μM SA. Conclusions: The biological activities of SA were explored for the first time. Our results stated that SA exhibited significant cytoproliferative and minor cytoprotective effects on HUVECs. We presume that the mechanisms of the proliferation and protection actions of SA involve interference with the generation of ROS and the cell apoptosis. These findings provide a new perspective on the biological potential of butenolides. View Full-Text

Keywords: striatisporolide A; Athyrium multidentatum Doell. Ching; butenolides; cytoprotective effect; ROS; apoptosis striatisporolide A; Athyrium multidentatum Doell. Ching; butenolides; cytoprotective effect; ROS; apoptosis





Autor: Dong-Mei Liu 1,†, Ji-Wen Sheng 1,†,* , Si-Hong Wang 2, Wei-Fen Zhang 1, Wei Zhang 3 and Dai-Juan Zhang 1

Fuente: http://mdpi.com/



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