Group I Intron Internal Guide Sequence Binding Strength as a Component of Ribozyme Network FormationReportar como inadecuado


Group I Intron Internal Guide Sequence Binding Strength as a Component of Ribozyme Network Formation


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Department of Chemistry, Portland State University, Portland, OR 97202, USA





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Academic Editor: Sabine Müller

Abstract Origins-of-life research requires searching for a plausible transition from simple chemicals to larger macromolecules that can both hold information and catalyze their own production. We have previously shown that some group I intron ribozymes possess the ability to help synthesize other ribozyme genotypes by recombination reactions in small networks in an autocatalytic fashion. By simplifying these recombination reactions, using fluorescent anisotropy, we quantified the thermodynamic binding strength between two nucleotides of two group I intron RNA fragments for all 16 possible genotype combinations. We provide evidence that the binding strength KD between the 3-nucleotide internal guide sequence IGS of one ribozyme and its complement in another is correlated to the catalytic ability of the ribozyme. This work demonstrates that one can begin to deconstruct the thermodynamic basis of information in prebiotic RNA systems. View Full-Text

Keywords: RNA; ribozymes; origins of life; internal guide sequence; recombination; fluorescence anisotropy RNA; ribozymes; origins of life; internal guide sequence; recombination; fluorescence anisotropy





Autor: Laura Elizabeth Satterwhite, Jessica A. M. Yeates and Niles Lehman *

Fuente: http://mdpi.com/



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