The Vital Dye CDr10b Labels the Zebrafish Mid-Intestine and LumenReportar como inadecuado


The Vital Dye CDr10b Labels the Zebrafish Mid-Intestine and Lumen


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1

Department of Molecular Medicine and Pathology, University of Auckland, Auckland 1142, New Zealand

2

Department of Chemistry and NUS MedChem Program of Life Sciences Institute, National University of Singapore and Singapore Bioimaging Consortium, A* STAR, Singapore 138667, Singapore





*

Author to whom correspondence should be addressed.



Academic Editor: Derek J. McPhee

Abstract We describe the use of the fluorescent reporter compound CDr10b to label mid-intestinal structures in zebrafish larvae after simple immersion. CDr10b is deposited into the gut where it initially fills the lumen and is excreted. Using laser-mediated injury of the intestine, we show that CDr10b provides a useful readout of the integrity and repair of the epithelial cell barrier. In addition, CDr10b specifically labels the absorptive mid-intestine segment that is analogous to the mammalian small intestine. By perturbing retinoic acid signaling, which regulates the size of the mid-intestine segment, we show that CDr10b is a valuable tool to rapidly assess developmental malformations of the intestine in live animals. View Full-Text

Keywords: zebrafish; gut development; mid-intestine; CDr10b; vital dye; laser ablation zebrafish; gut development; mid-intestine; CDr10b; vital dye; laser ablation





Autor: Veronika Sander 1, Shantanu Patke 1, Jung Y. Lee 2, Young-Tae Chang 2 and Alan J. Davidson 1,*

Fuente: http://mdpi.com/



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