Ginsenoside PPD’s Antitumor Effect via Down-Regulation of mTOR Revealed by Super-Resolution ImagingReportar como inadecuado


Ginsenoside PPD’s Antitumor Effect via Down-Regulation of mTOR Revealed by Super-Resolution Imaging


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1

Department of Otolaryngology Head and Neck Surgery, The Second Hospital, Jilin University, Changchun 13041, Jilin, China

2

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 13021, Jilin, China

3

School of Nursing, Jilin University, Changchun 13021, Jilin, China

4

Department of Immunology, University of Pittsburgh, Pittsburgh, PA 15261, USA



These authors contributed equally to this work.





*

Author to whom correspondence should be addressed.



Academic Editor: Derek J. McPhee

Abstract Derived from Panax ginseng, the natural product 20S-Protopanaxadiol PPD has been reported for its cytotoxicity against several cancer cell lines. The molecular mechanism is, however, not well understood. Here we show that PPD significantly inhibits proliferation, induces apoptosis and causes G2-M cell cycle arrest in human laryngeal carcinoma cells Hep-2 cells. PPD also decreases the levels of proteins related to cell proliferation. Moreover, PPD-induced apoptosis is characterized by a dose-dependent down-regulation of Bcl-2 expression and up-regulation of Bax, and is accompanied by the activation of Caspase-3 as well. Further molecular mechanism is revealed by direct stochastic optical reconstruction microscopy dSTORM—a novel high-precision localization microscopy which enables effective resolution down to the order of 10 nm. It shows the expression and spatial arrangement of mTOR and its downstream effectors, demonstrating that this ginsenoside exerts its excellent anticancer effects via down-regulation of mTOR signaling pathway in Hep-2 cells. Taken together, our findings elucidate that the antitumor effect of PPD is associated with its regulation of mTOR expression and distribution, which encourages further studies of PPD as a promising therapeutic agent against laryngeal carcinoma. View Full-Text

Keywords: PPD; laryngeal cancer; mTOR; antitumor; dSTORM PPD; laryngeal cancer; mTOR; antitumor; dSTORM





Autor: Bo Teng 1,†,* , Junguang Jiang 2,†, Lijing Zhao 3,†, Jing Gao 2, Junyu Chen 1, Zhe Liu 1, Hongda Wang 2 and Binfeng Lu 4

Fuente: http://mdpi.com/



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