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1

Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44-52, 01-224 Warsaw, Poland

2

Faculty of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland

3

Department of Biophysics, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, Poland





*

Author to whom correspondence should be addressed.



Academic Editors: Andrew deMello and Xavier Casadevall i Solvas

Abstract We demonstrate a technique that uses microdroplets for culturing and selecting bacterial cultures in a model biotechnological application. We propose an assay for determination of ethanol concentration that provides increased dynamic range and is compatible with droplet microfluidic screening technologies. The assay comprises two enzymes—alcohol oxidase AOX and horseradish peroxidase HRP—and a colorimetric readout system of phenol-4-sulfonic acid PSA and 4-aminoantipyrine 4-AAP. The microdroplet method provides high repeatability a relative error of measured ethanol concentration < 5%, high specificity for ethanol, low consumption of reagents and wide dynamic range 1–70 g·L-1 compared to existing assays. We report the use of this method in a screen of ethanol generation efficiency of Zymomonas mobilis strain 3881 against the concentration of glucose in the culture media. View Full-Text

Keywords: droplet microfluidics; cell culturing; ethanol fermentation; enzymatic assay; process optimization droplet microfluidics; cell culturing; ethanol fermentation; enzymatic assay; process optimization





Autor: Krzysztof Churski 1,2, Artur Ruszczak 1, Slawomir Jakiela 1,3 and Piotr Garstecki 1,*

Fuente: http://mdpi.com/



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