Comparison of the Influence of Phospholipid-Coated Porous Ti-6Al-4V Material on the Osteosarcoma Cell Line Saos-2 and Primary Human Bone Derived CellsReportar como inadecuado




Comparison of the Influence of Phospholipid-Coated Porous Ti-6Al-4V Material on the Osteosarcoma Cell Line Saos-2 and Primary Human Bone Derived Cells - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Institute of Materials Research, Division of Metallic Biomaterials, Helmholtz-Zentrum Geesthacht, Geesthacht 21502, Germany





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Academic Editor: Hugo F. Lopez

Abstract Biomaterial surface functionalization remains of great interest in the promotion of cell osteogenic induction. Previous studies highlighted the positive effects of porous Ti-6Al-4V and phospholipid coating on osteoblast differentiation and bone remodeling. Therefore, the first objective of this study was to evaluate the potential synergistic effects of material porosity and phospholipid coating. Primary human osteoblasts and Saos-2 cells were cultured on different Ti-6Al-4V specimens mirror-like polished or porous specimens and were coated or not with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine POPE for three weeks or five weeks. Selected gene expressions e.g., classical bone markers: alkaline phosphatase, osteocalcin, osteoprotegerin OPG, receptor activator of nuclear factor kappa-β ligand RANKL and runt-related transcription factor 2 were estimated in vitro. Furthermore, the expressions of osteocalcin and osteopontin were examined via fluorescent microscopy at five weeks immunocytochemistry. Consequently, it was observed that phospholipid coating potentiates preferences for low and high porosities in Saos-2 and primary cells, respectively, at the gene and protein levels. Additionally, RANKL and OPG exhibited different gene expression patterns; primary cells showed dramatically increased RANKL expression, whereas OPG expression was decreased in the presence of POPE. A synergistic effect of increased porosity and phospholipid coating was observed in primary osteoblasts in bone remodeling. This study showed the advantage of primary cells over the standard bone cell model. View Full-Text

Keywords: Ti-6Al-4V; biomaterial; porous; implant; phospholipid; biomimetic coating; immunocytochemistry; gene expression Ti-6Al-4V; biomaterial; porous; implant; phospholipid; biomimetic coating; immunocytochemistry; gene expression





Autor: Axel Deing, Thomas Ebel, Regine Willumeit-Römer and Bérengère J.C. Luthringer *

Fuente: http://mdpi.com/



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