Methanol Extract from Anogeissus leiocarpus DC Guill. et Perr. Combretaceae Stem Bark Quenches the Quorum Sensing of Pseudomonas aeruginosa PAO1Reportar como inadecuado


Methanol Extract from Anogeissus leiocarpus DC Guill. et Perr. Combretaceae Stem Bark Quenches the Quorum Sensing of Pseudomonas aeruginosa PAO1


Methanol Extract from Anogeissus leiocarpus DC Guill. et Perr. Combretaceae Stem Bark Quenches the Quorum Sensing of Pseudomonas aeruginosa PAO1 - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Laboratoire de Biochimie & Chimie Appliquées, Université Ouaga 1 Pr. Joseph KI‐ZERBO, Ouagadougou, 03 BP 7021, Burkina Faso





*

Author to whom correspondence should be addressed.



Abstract Background: Due to its extensive arsenal of virulence factors and inherent resistance to antibiotics, Pseudomonas aeruginosa is a threat particularly in immunocompromised patients. Considering the central role of quorum sensing in the production of virulence factors, inhibition of bacterial communication mechanism constitute an opportunity to attenuate pathogenicity of bacteria resistant to available antibiotics. Our study aimed to assess the anti-quorum sensing activity of Anogeissus leiocarpus, traditionally used in Burkina Faso, for the treatment of infected burn wounds. Methods: Investigations were carried out on methanol extract from A. leiocarpus stem bark. The reporter strains Chromobacterium violaceum CV026 and P. aeruginosa PAO1 derivatives were used to evidence any interference with the bacterial quorum sensing and expression of related genes. P. aeruginosa PAO1 was used to measure the impact on pyocyanin production. Results: At a sub-inhibitory concentration 100 µg-mL, A. leiocarpus methanol extract quenched the quorum sensing mechanism of P. aeruginosa PAO1 by down-streaming the rhlR gene, with a subsequent reduction of pyocyanin production. Moreover, the antioxidant polyphenols evidenced are able to reduce the oxidative stress induced by pyocyanin. Conclusion: The antioxidant and anti-quorum sensing activities of A. leiocarpus stem bark could justify its traditional use in the treatment of infected burn wounds. View Full-Text

Keywords: Anogeissus leiocarpus; Chromobacterium violaceum CV026; Pseudomonas aeruginosa PAO1; pyocyanin; quorum sensing Anogeissus leiocarpus; Chromobacterium violaceum CV026; Pseudomonas aeruginosa PAO1; pyocyanin; quorum sensing





Autor: Vincent Ouedraogo and Martin Kiendrebeogo *

Fuente: http://mdpi.com/



DESCARGAR PDF




Documentos relacionados