Vochysia rufa Stem Bark Extract Protects Endothelial Cells against High Glucose DamageReportar como inadecuado


Vochysia rufa Stem Bark Extract Protects Endothelial Cells against High Glucose Damage


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1

Department of Biofunctional Science, Faculty Morgana Potrich, Mineiros, Goias 75830-000, Brazil

2

Department of Metabolism and Nutrition, Institute of Food Science, Technology and Nutrition ICTAN–CSIC, Madrid 28040, Spain

3

Institute of Genetics and Biochemistry, Federal University of Uberlandia, Uberlandia, Minas Gerais 38400-712, Brazil

4

Department of Pharmacology, Faculty of Pharmacy, Universidad Complutense de Madrid, Madrid 28040, Spain





*

Author to whom correspondence should be addressed.



Academic Editor: João Rocha

Abstract Background: Increased oxidative stress by persistent hyperglycemia is a widely accepted factor in vascular damage responsible for type 2 diabetes complications. The plant Vochysia rufa Vr has been used in folk medicine in Brazil for the treatment of diabetes. Thus; the protective effect of a Vr stem bark extract against a challenge by a high glucose concentration on EA.hy926 EA endothelial cells is evaluated. Methods: Vegetal material is extracted with distilled water by maceration and evaporated until dryness under vacuum. Then; it is isolated by capillary electrophoresis–tandem mass spectrometry. Cell viability is evaluated on EA cells treated with 0.5–100 µg-mL of the Vr extract for 24 h. The extract is diluted at concentrations of 5, 10 and 25 µg-mL and maintained for 24 h along with 30 mM of glucose to evaluate its protective effect on reduced glutathione GSH; glutathione peroxidase GPx and reductase GR and protein carbonyl groups. Results: V. rufa stem bark is composed mainly of sugars; such as inositol; galactose; glucose; mannose; sacarose; arabinose and ribose. Treatment with Vr up to 100 µg-mL for 24 h did not affect cell viability. Treatment of EA cells with 30 mM of glucose for 24 h significantly increased the cell damage. EA cells treated with 30 mM of glucose showed a decrease of GSH concentration and increased Radical Oxygen Species ROS and activity of antioxidant enzymes and protein carbonyl levels; compared to control. Co-treatment of EA with 30 mM glucose plus 1–10 μg-mL Vr significantly reduced cell damage while 5–25 μg-mL Vr evoked a significant protection against the glucose insult; recovering ROS; GSH; antioxidant enzymes and carbonyls to baseline levels. Conclusion: V. rufa extract protects endothelial cells against oxidative damage by modulating ROS; GSH concentration; antioxidant enzyme activity and protein carbonyl levels. View Full-Text

Keywords: Vochysia rufa; Sapotaceae; endothelial cells; oxidative damage; plant antioxidants Vochysia rufa; Sapotaceae; endothelial cells; oxidative damage; plant antioxidants





Autor: Neire Moura de Gouveia 1, Sonia Ramos 2, María Ángeles Martín 2, Foued Salmen Espindola 3, Luis Goya 2 and Olga M. Palomino 4,*

Fuente: http://mdpi.com/



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