Neural Stem Cell Spreading on Lipid Based Artificial Cell Surfaces, Characterized by Combined X-ray and Neutron ReflectometryReportar como inadecuado


Neural Stem Cell Spreading on Lipid Based Artificial Cell Surfaces, Characterized by Combined X-ray and Neutron Reflectometry


Neural Stem Cell Spreading on Lipid Based Artificial Cell Surfaces, Characterized by Combined X-ray and Neutron Reflectometry - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

1

Ludwig-Maximilians-Universität, Department für Physik and CeNS, Geschwister-Scholl-Platz 1, 80539 Munich, Germany

2

Research Group of Peptide Chemistry, Hungarian Academy of Science, Eötvös L. University, Pazmany P. stny. 1-A, 1117 Budapest, Hungary

3

Laboratory of Cellular and Developmental Neurobiology, Institute of Experimental Medicine of Hungarian Academy of Science, Szigony u. 43, Budapest, H-1083, Hungary





*

Author to whom correspondence should be addressed.



Abstract We developed a bioadhesive coating based on a synthetic peptide-conjugate AK-cycloRGDfC which contains multiples of the arginyl-glycyl-aspartic acid RGD amino acid sequence. Biotinylated AK-cycloRGDfC is bound to a supported lipid bilayer via a streptavidin interlayer. Layering, hydration and packing of the coating is quantified by X-ray and neutron reflectometry experiments. AK-cycloRGDfC binds to the streptavidin interlayer in a stretched-out on edge configuration. The highly packed configuration with only 12% water content maximizes the number of accessible adhesion sites. Enhanced cell spreading of neural stem cells was observed for AK-cycloRGDfC functionalized bilayers. Due to the large variety of surfaces which can be coated by physisorption of lipid bilayers, this approach is of general interest for the fabrication of biocompatible surfaces. View Full-Text

Keywords: supported lipid bilayers; biomimetics; bionanotechnology; functional coatings; self-assembly supported lipid bilayers; biomimetics; bionanotechnology; functional coatings; self-assembly





Autor: Martin Huth 1, Samira Hertrich 1, Gabor Mezo 2, Emilia Madarasz 3 and Bert Nickel 1,*

Fuente: http://mdpi.com/



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