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1

School of Dentistry, College of Oral Medicine, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan

2

School of Dental Technology, College of Oral Medicine, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan

3

Graduate Institute of Biomedical Materials & Tissue Engineering, College of Oral Medicine, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan

4

Dental Department of Taipei Medical University, Shuang-Ho Hospital, Taipei 110, Taiwan



These authors contributed equally to this work.





*

Author to whom correspondence should be addressed.



Academic Editor: Franz E. Weber

Abstract Bone regeneration procedures require alternative graft biomaterials to those for autogenous bone. Therefore, we developed a novel porcine graft using particle sizes of 250–500 μm and 500–1000 μm in rabbit calvarial bone defects and compared the graft properties with those of commercial hydroxyapatite HA-beta-tricalcium phosphate β-TCP over eight weeks. Surgery was performed in 20 adult male New Zealand white rabbits. During a standardized surgical procedure, four calvarial critical-size defects of 5 mm diameter and 3 mm depth were prepared. The defects were filled with HA-β-TCP, 250–500 μm or 500–1000 μm porcine graft, and control defects were not filled. The animals were grouped for sacrifice at 1, 2, 4, and 8 weeks post-surgery. Subsequently, sample blocks were prepared for micro-computed tomography micro-CT scanning and histological sectioning. Similar bone formations were observed in all three treatment groups, although the 250–500 μm porcine graft performed slightly better. Rabbit calvarial bone tissue positively responded to porcine grafts and commercial HA-β-TCP, structural analyses showed similar crystallinity and porosity of the porcine and HA-β-TCP grafts, which facilitated bone formation through osteoconduction. These porcine grafts can be considered as graft substitutes, although further development is required for clinical applications. View Full-Text

Keywords: bone regeneration; bone graft; porcine tissue; in vivo test; hydroxyapatite bone regeneration; bone graft; porcine tissue; in vivo test; hydroxyapatite





Autor: Eisner Salamanca 1,†, Wei-Fang Lee 2,†, Chin-Yi Lin 1, Haw-Ming Huang 3, Che-Tong Lin 1, Sheng-Wei Feng 1 and Wei-Jen Chang 1,4,*

Fuente: http://mdpi.com/



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