Application of a Loop-Type Laboratory Biofilm Reactor to the Evaluation of Biofilm for Some Metallic Materials and Polymers such as Urinary Stents and CathetersReportar como inadecuado


Application of a Loop-Type Laboratory Biofilm Reactor to the Evaluation of Biofilm for Some Metallic Materials and Polymers such as Urinary Stents and Catheters


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1

Department of Materials Science and Engineering, National Institute of Technology, Suzuka College, Suzuka Mie 510-0294, Japan

2

Department of Chemistry and Biochemistry, National Institute of Technology, Suzuka College, Suzuka Mie 510-0294, Japan





*

Author to whom correspondence should be addressed.



Academic Editor: Jun-ichi Anzai

Abstract A laboratory biofilm reactor LBR was modified to a new loop-type closed system in order to evaluate novel stents and catheter materials using 3D optical microscopy and Raman spectroscopy. Two metallic specimens, pure nickel and cupronickel 80% Cu-20% Ni, along with two polymers, silicone and polyurethane, were chosen as examples to ratify the system. Each set of specimens was assigned to the LBR using either tap water or an NB Nutrient broth based on peptone from animal foods and beef extract mainly—cultured solution with E-coli formed over 48–72 h. The specimens were then analyzed using Raman Spectroscopy. 3D optical microscopy was employed to corroborate the Raman Spectroscopy results for only the metallic specimens since the inherent roughness of the polymer specimens made such measurements difficult. The findings suggest that the closed loop-type LBR together with Raman spectroscopy analysis is a useful method for evaluating biomaterials as a potential urinary system. View Full-Text

Keywords: biofilm; loop-type laboratory biofilm reactor; Raman spectroscopy biofilm; loop-type laboratory biofilm reactor; Raman spectroscopy





Autor: Hideyuki Kanematsu 1,* , Hikonaru Kudara 1, Shun Kanesaki 1, Takeshi Kogo 1, Hajime Ikegai 2, Akiko Ogawa 2 and Nobumitsu Hirai 2

Fuente: http://mdpi.com/



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