Behavior of Human Bone Marrow-Derived Mesenchymal Stem Cells on Various Titanium-Based CoatingsReportar como inadecuado


Behavior of Human Bone Marrow-Derived Mesenchymal Stem Cells on Various Titanium-Based Coatings


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1

Department of Orthopaedics, Traumatology and Hand Surgery, Kuopio University Hospital, Kuopio 70210, Finland

2

Department of Integrative Medical Biology, Umeå University, Umeå 90187, Sweden

3

Department of Applied Physics, University of Eastern Finland, Kuopio 70211, Finland

4

Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, School of Public Health of Health Science Center, Xi’an Jiaotong University, Xi’an 710061, China





*

Authors to whom correspondence should be addressed.



Academic Editor: Georgios E. Romanos

Abstract The chemical composition and texture of titanium coatings can influence the growth characteristics of the adhered cells. An enhanced proliferation of the human mesenchymal stem cells hMSCs would be beneficial. The present study was aimed to investigate whether titanium deposited at different atmospheres would affect the cell growth properties, cellular morphology, and expression of surface markers of hMSCs. Titanium-based coatings were deposited on silicon wafers under oxygen, nitrogen, or argon atmospheres by ultra-short pulsed laser deposition using two different gas pressures followed by heating at 400 °C for 2 h. The characteristics of the coated surfaces were determined via contact angle, zeta potential, and scanning electron microscopy SEM techniques. Human MSCs were cultivated on differently coated silicon wafers for 48 h. Subsequently, the cell proliferation rates were analyzed with an MTT assay. The phenotype of hMSCs was checked via immunocytochemical stainings of MSC-associated markers CD73, CD90, and CD105, and the adhesion, spreading, and morphology of hMSCs on coated materials via SEM. The cell proliferation rates of the hMSCs were similar on all coated silicon wafers. The hMSCs retained the MSC phenotype by expressing MSC-associated markers and fibroblast-like morphology with cellular projections. Furthermore, no significant differences could be found in the size of the cells when cultured on all various coated surfaces. In conclusion, despite certain differences in the contact angles and the zeta potentials of various titanium-based coatings, no single coating markedly improved the growth characteristics of hMSCs. View Full-Text

Keywords: human bone marrow-derived mesenchymal stem cells; ultra-short pulsed laser deposition; surface coating; cell culture; adhesion behavior human bone marrow-derived mesenchymal stem cells; ultra-short pulsed laser deposition; surface coating; cell culture; adhesion behavior





Autor: Chengjuan Qu 1,2,* , Salla Kaitainen 3, Heikki Kröger 1, Reijo Lappalainen 3 and Mikko J. Lammi 2,4,*

Fuente: http://mdpi.com/



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