Synthesis and Characterization of PolyEthylene Glycol Based Thermo-Responsive Hydrogels for Cell Sheet EngineeringReportar como inadecuado


Synthesis and Characterization of PolyEthylene Glycol Based Thermo-Responsive Hydrogels for Cell Sheet Engineering


Synthesis and Characterization of PolyEthylene Glycol Based Thermo-Responsive Hydrogels for Cell Sheet Engineering - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

1

Department of Thoracic and Cardiovascular Surgery, Gachon University Gil Medical Center, Gachon University, 34, Namdong-daero 774beon-gil, Namdong-gu, Incheon 21565, Korea

2

Department of Molecular Medicine, School of Medicine, Gachon University, 155 Gaetbeol-ro, Yeonsu-ku, Incheon 21999, Korea





*

Author to whom correspondence should be addressed.



Academic Editor: Jie Zheng

Abstract The swelling properties and thermal transition of hydrogels can be tailored by changing the hydrophilic-hydrophobic balance of polymer networks. Especially, polyN-isopropylacrylamide PNIPAm has received attention as thermo-responsive hydrogels for tissue engineering because its hydrophobicity and swelling property are transited around body temperature 32 °C. In this study, we investigated the potential of polyethylene glycol diacrylate PEGDA as a hydrophilic co-monomer and crosslinker of PNIPAm to enhance biological properties of PNIPAm hydrogels. The swelling ratios, lower critical solution temperature LCST, and internal pore structure of the synthesized pNIPAm-co-PEGDA hydrogels could be varied with changes in the molecular weight of PEGDA and the co-monomer ratios NIPAm to PEGDA. We found that increasing the molecular weight of PEGDA showed an increase of pore sizes and swelling ratios of the hydrogels. In contrast, increasing the weight ratio of PEGDA under the same molecular weight condition increased the crosslinking density and decreased the swelling ratios of the hydrogels. Further, to evaluate the potential of these hydrogels as cell sheets, we seeded bovine chondrocytes on the pNIPAm-co-PEGDA hydrogels and observed the proliferation of the seed cells and their detachment as a cell sheet upon a decrease in temperature. Based on our results, we confirmed that pNIPAm-co-PEGDA hydrogels could be utilized as cell sheets with enhanced cell proliferation performance. View Full-Text

Keywords: N-isopropylacrylamide NIPAm; polyethylene glycol diacrylate PEGDA; thermo-responsive hydrogel; cell sheet; tissue engineering N-isopropylacrylamide NIPAm; polyethylene glycol diacrylate PEGDA; thermo-responsive hydrogel; cell sheet; tissue engineering





Autor: Kuk Hui Son 1 and Jin Woo Lee 2,*

Fuente: http://mdpi.com/



DESCARGAR PDF




Documentos relacionados