Gold Nanoparticle-Based Colorimetric and Electrochemical Methods for Dipeptidyl Peptidase-IV Activity Assay and Inhibitor ScreeningReportar como inadecuado


Gold Nanoparticle-Based Colorimetric and Electrochemical Methods for Dipeptidyl Peptidase-IV Activity Assay and Inhibitor Screening


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Henan Province of Key Laboratory of New Optoelectronic Functional Materials, College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, Henan, China





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Academic Editor: Ilaria Fratoddi

Abstract We presented the colorimetric and electrochemical methods for determination of the dipeptidyl peptidase-IV DPP-IV activity and screening of its inhibitor using gold nanoparticle AuNP as the probe. In the colorimetric assay, the substrate peptide with a sequence of Arg-Pro-Arg induced the aggregation and color change of AuNPs, whereas cleavage of the peptide by DPP-IV prevented the aggregation of AuNPs. Furthermore, the aggregation of AuNPs in the solution was easily initiated on a solid-liquid electrode-electrolyte surface, which induced a decrease in the electron-transfer resistance. However, once the peptide was clipped by DPP-IV, the assembly of AuNPs on electrode surface was prevented. Consequently, a higher electron-transfer resistance was observed. The colorimetric and electrochemical assays allowed for the determination of DPP-IV with the detection limits of 70 μU-mL and 0.55 μU-mL, respectively. Meanwhile, the proposed methods were used to determine DPP-IV inhibitor with satisfactory results. Both the colorimetric and electrochemical methods are simple, rapid and sufficiently sensitive for DPP-IV activity assay and inhibitor screening. The results also demonstrated that the AuNP-based colorimetric assay could be converted into an enhanced surface tethered electrochemical assay with improving sensitivity. The simple detection principle may be extended to the design of other peptidases biosensors with easy manipulation procedures. View Full-Text

Keywords: dipeptidyl peptidase-IV; gold nanoparticles; colorimetric assay; electrochemical impedance spectroscopy; inhibitor screening dipeptidyl peptidase-IV; gold nanoparticles; colorimetric assay; electrochemical impedance spectroscopy; inhibitor screening





Autor: Ning Xia * , Xin Wang, Xiaojin Wang and Binbin Zhou

Fuente: http://mdpi.com/



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